An attenuating role of a WASP-related protein, WASP-B, in the regulation of F-actin polymerization and pseudopod formation via the regulation of RacC during Dictyostelium chemotaxis.
An attenuating role of a WASP-related protein, WASP-B, in the regulation of F-actin polymerization and pseudopod formation via the regulation of RacC during Dictyostelium chemotaxis.
复制标题
WASP 相关蛋白 WASP-B 在盘基网柄菌趋化过程中通过调节 RacC 在调节 F-肌动蛋白聚合和伪足形成中发挥减弱作用。
DOI：
10.1016/j.bbrc.2013.06.022
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发表时间：
2013
影响因子：
3.1
通讯作者：
Mandel,NicoleS
中科院分区：
文献类型：
--
作者：
Chung,ChangY;Feoktistov,Alexander;Hollingsworth,RyanJ;Rivero,Francisco;Mandel,NicoleS
The WASP family of proteins has emerged as important regulators that connect multiple signaling pathways to regulate the actin cytoskeleton.Dictyosteliumcells express WASP, as well as a WASP related protein, WASP-B, endoded bywasBgene. WASP-B contains many of the domains present in WASP. Analysis of wild type,wasBnull cells revealed that WASP-B is required for proper control of F-actin polymerization in response to a cAMP gradient. Due to the lack of tight control on actin polymerization,wasBnull cells exhibited higher level of F-actin polymerization.wasB−cells extend morede novopseudopods laterally and their average life span is longer than those of wild type cells, causing more turns and inefficient chemotaxis. YFP-WASP-B appears to be uniformly distributed in the cytosol and shows no translocation to cortical membrane upon cAMP stimulation. Active RacC pull-down assay reveals that the level of active RacC inwasB−cells is significantly higher than wild type cells. Moreover, the distribution of active RacC is not localized inwasB−cells.We conclude that chemotaxis defects ofwasB−cells are likely to result from the aberrant regulation of RacC activation and localization.
