Measurement of the shrinkage of natural and simulated lesions on root surfaces using CP-OCT.
Measurement of the shrinkage of natural and simulated lesions on root surfaces using CP-OCT.
复制标题
使用 CP-OCT 测量根表面自然和模拟病变的收缩。
DOI：
10.1016/j.jdent.2019.103213
复制
发表时间：
2019
影响因子：
4.4
通讯作者：
Fried,Daniel
中科院分区：
文献类型：
--
作者：
Yang,Vincent;Fried,Daniel
目的脱矿根、牙本质和牙骨质主要由胶原蛋白组成，胶原蛋白在脱水或风干后会显著收缩。在再矿化过程中，矿物质沉积在病变的外部，形成一层高度矿化的表层，阻止扩散，阻止病变并防止收缩。以前的研究表明，活跃的根面龋损表现出萎缩，而停滞的病变在脱水后不再表现出萎缩。本研究的目的是证明在脱水过程中可以使用适合临床使用的光学相干断层扫描探头来监测根面龋损的收缩。方法在体外研究中，使用交叉偏振光学相干断层扫描(CP-OCT)系统和适合临床使用的带有集成空气喷嘴的3D打印矫治器来测量模拟根面龋损和自然根面龋损在拔除牙齿上的收缩。结果CP-OCT成功地测量了病变表面的显著收缩(P <； 0.0 5)，病变厚度显著减小，每微米反射率显著增加。结论在这项临床前研究中，我们证明了一种改进的感染控制CP-OCT手持器可以用于监测根面龋损的缩小。此外，我们开发了一种使用OCT测量病变缩小的新方法，即监控病变厚度的变化，而不是病变表面的位置，该方法不需要初始参考位置，更容易在体内实现。
ObjectivesDemineralized root dentin and cementum is made up of mostly collagen that shrinks significantly upon dehydration or drying with air. During remineralization mineral is deposited on the outside of the lesion creating a highly mineralized surface layer that inhibits diffusion, arrests the lesion and prevents shrinkage. Previous studies suggest that active root caries lesions manifest shrinkage, while arrested lesions no longer manifest shrinkage upon dehydration. The purpose of this study was to demonstrate that the shrinkage of root caries lesions can be monitored during dehydration using an optical coherence tomography probe suitable for clinical use.MethodsIn thisin vitrostudy the shrinkage of simulated and natural root caries lesions on extracted teeth was measured using a cross polarization optical coherence tomography (CP-OCT) system and a 3D printed appliance with an integrated air nozzle suitable for clinical use. Two methods were employed to assess shrinkage, changes in the position of the lesion surface and changes in the thickness of the lesion.ResultsCP-OCT was successful in measuring a significant (P < 0.05) contraction of the lesion surface, significant decrease in the lesion thickness and increase in the reflectivity per micron upon drying natural lesions on extracted teeth.ConclusionsIn this preclinical study, we have demonstrated that a CP-OCT handpiece modified for infection control with an attached air nozzle suitable forin vivouse can be used to monitor the shrinkage of root caries lesions. In addition, we have developed a new approach to measuring lesion shrinkage with OCT, namely monitoring changes in the lesion thickness as opposed to the position of the lesion surface, that does not require an initial reference position and is more easily implementedin vivo.