Effect of feeding different types of β-glucans derived from two marine diatoms (Chaetoceros muelleri and Thalassiosira weissflogii) on growth performance and immunity of banana shrimp (Penaeus merguiensis)
Effect of feeding different types of β-glucans derived from two marine diatoms (Chaetoceros muelleri and Thalassiosira weissflogii) on growth performance and immunity of banana shrimp (Penaeus merguiensis)
复制标题
饲喂两种海洋硅藻（Muelleri 角毛藻和 Weissflogii 海藻）来源的不同类型 β-葡聚糖对香蕉虾（Penaeus merguiensis）生长性能和免疫力的影响
DOI：
10.1016/j.fsi.2022.09.047
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发表时间：
2022
影响因子：
4.7
通讯作者：
Wuthisuthimethavee Suwit
中科院分区：
文献类型：
--
作者：
Pooljun Chettupon;Jariyapong Pitchanee;Wongtawan Tuempong;Hirono Ikuo;Wuthisuthimethavee Suwit
β-葡聚糖由许多生物产生，可作为补充饲料，提高某些水生动物的免疫力和生长。本研究旨在比较两种海洋硅藻（muellerichaetoceros muellerios和weissflogithalassisira）中提取的β-葡聚糖作为香蕉对虾（Penaeus merguiensis）生长促进剂和免疫增强剂的效果。将对虾分为3组：对照组饲喂不添加β-葡聚糖；第二组和第三组分别饲喂2 g kg−1的β-葡聚糖。muelleriandT。分别weissflogii。饲喂30 d，分别在第15天和第30天测定虾的生长性能（末重、增重、平均日增重和饲料系数）。测定免疫参数为总血细胞计数（THC）、酚氧化酶活性（PO）和免疫基因表达。在饲养试验14 d和感染副溶血性弧菌（感染后6、24、48 h）后测定存活率。两种β-葡聚糖对对虾生长的刺激作用无显著差异（P < 0.05）。muelleriorT。weissflogii)。其中，β-葡聚糖组的最终体重、增重和平均日增重均高于对照组（P< 0.05），而两种β-葡聚糖组的FCR均低于对照组。β-葡聚糖，尤其是来自mc的β-葡聚糖，显著提高了对虾的免疫参数、THC、PO以及抗脂多糖因子（ALF）和硬壳蛋白的基因表达量（P< 0.05）。治疗前后均明显高于对照组。parahaemolyticusinfection。细菌感染β-葡聚糖后，对虾中对虾苷3和过氧化物还氧蛋白基因的表达显著升高。肝胰腺组织病理学结果显示，饲养14天后肝胰腺blasenen上皮细胞（B细胞）增加，感染v病毒后仍保持较高水平。parahaemolyticus。用含有β-葡聚糖的饲料喂养虾的存活率。muelleri(82.2%)支持。weissflogii afterV(77.8%)。溶血性副溶血感染率显著高于对照组（51.1%）（P< 0.05）。综上所述，我们建议用从海洋硅藻中提取的β-葡聚糖饲喂香蕉虾。muelleriorT。2 g kg - 1的饲粮可显著提高魏氏肉鸡的生长性能和免疫力。
β-glucans are produced by many organisms and could be used as supplementary feed to enhance immunity and growth in some aquatic animals. This study aimed to compare the effectiveness of β-glucans derived from two marine diatoms (Chaetoceros muelleriandThalassiosira weissflogii) as growth promoters and immunity enhancers in banana shrimp (Penaeus merguiensis). Shrimp were divided into 3 groups: the control group was fed without β-glucan; the second and the third group were fed with 2 g kg−1of β-glucan derived fromC. muelleriandT. weissflogii, respectively. Shrimp were fed over a 30-day period to determine growth performance (final weight, weight gain, average daily gain (ADG), and feed conversion ratio (FCR)) at day 15 and day 30, respectively. The immune parameters determined were total hemocyte count (THC), phenoloxidase activity (PO) and immune gene expression. Survival rates were measured after 14 days of the feeding trial andVibrio parahaemolyticusinfection (6, 24, 48 h post infection). There was no significant difference (P> 0.05) for growth stimulation of shrimps between the two types of β-glucans(C. muelleriorT. weissflogii). Notably, shrimps fed with β-glucans had a higher final weight, weight gain, and ADG (P< 0.05) than shrimps fed with the control diet, while FCR of shrimps fed with both β-glucans was lower when compared to the control diet. Immune parameters, THC, PO, and gene expression of anti-lipopolysaccharide factor (ALF) and crustin were significantly higher (P< 0.05) in shrimps fed with β-glucans, especially with β-glucans fromC. muellerithan the control group both before and afterV. parahaemolyticusinfection. Expression of penaeidin 3 and peroxiredoxin genes was significantly higher in shrimps fed with β-glucans after bacterial infection. Histopathology of hepatopancreas revealed an increase in blasenzellen hepatopancreatic epithelial cells (B cells) after 14 days of feeding which remained higher following infection withV. parahaemolyticus. The survival rate of shrimps fed with the diet containing β-glucan derived from eitherC. muelleri(82.2%) orT. weissflogii(77.8%) afterV. parahaemolyticusinfection was significantly higher than for the control group (51.1%) (P< 0.05). In conclusion, we propose that feeding banana shrimps with β-glucans derived from marine diatoms eitherC. muelleriorT. weissflogiiat a 2 g kg−1diet can significantly improve their growth performance and immunity.