Effects of Concentration and Temperature of Nutrient Solution on Growth and Camptothecin Accumulation of Ophiorrhiza pumila
Effects of Concentration and Temperature of Nutrient Solution on Growth and Camptothecin Accumulation of Ophiorrhiza pumila
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DOI：
10.3390/plants9060793
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发表时间：
2020-06-01
期刊：
影响因子：
4.5
通讯作者：
Goto, Eiji
中科院分区：
文献类型：
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作者：
Lee, Ji-Yoon;Hiyama, Miki;Goto, Eiji
药用植物，蛇根草，自然生长在亚热带地区潮湿的内陆森林的地板上。它在所有器官中积累喜树碱（CPT），喜树碱用作抗肿瘤剂。研究了O.在一个植物工厂里。试验1以O.在开始处理（DAT）后，使用四种浓度（0.125、0.25、0.5和1.0倍）的商业溶液培养pumila 63天。这些神经干细胞的电导率分别为0.6、0.9、1.5和2.7 dS m（-1）。0.25和0.5 NSC组的总干重高于其他两组。0.25 NSC组CPT含量显著高于其他NSC组。试验二以O.将pumila在4个NST（10、20、26和35 ℃，分别命名为T10、T20、T26和T36）下培养35 DT，T20的生长量和CPT含量是各处理中最高的。因此，0.25 NSC和20 ℃ NST的根区环境产生了最好的生长和CPT积累在O。pumila。
The medicinal plant,Ophiorrhiza pumila, naturally grows on the floors of humid inland forests in subtropical areas. It accumulates camptothecin (CPT), which is used as an anti-tumor agent, in all organs. We investigated the optimal hydroponic root-zone environments for growth and CPT accumulation inO. pumilain a plant factory. In experiment 1, to determine the appropriate nutrient solution concentration (NSC),O. pumilawas cultivated using four concentrations (0.125, 0.25, 0.5, and 1.0 times) of a commercial solution for 63 days after the start of treatment (DAT). The electrical conductivity of these NSCs was 0.6, 0.9, 1.5, and 2.7 dS m(-1), respectively. The total dry weights at 0.25 and 0.5 NSCs were higher than those at the other two NSCs. CPT content at 0.25 NSC was significantly higher than those at other NSCs. In experiment 2, to investigate an appropriate nutrient solution temperature (NST),O. pumilawas cultivated at four NSTs (10, 20, 26, and 35 degrees C, named as T10, T20, T26, and T36, respectively) for 35 DAT. The growth and CPT content at T20 was the highest among the treatments. Therefore, root-zone environments of 0.25 NSC and 20 degrees C of NST produced the best growth and CPT accumulation inO. pumila.