METABOLISM OF C-MYC GENE-PRODUCTS - C-MYC MESSENGER-RNA AND PROTEIN EXPRESSION IN THE CELL-CYCLE
METABOLISM OF C-MYC GENE-PRODUCTS - C-MYC MESSENGER-RNA AND PROTEIN EXPRESSION IN THE CELL-CYCLE
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DOI：
10.1002/j.1460-2075.1985.tb03885.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
RABBITTS, TH
中科院分区：
文献类型：
--
作者：
RABBITTS, PH;WATSON, JV;RABBITTS, TH
研究c-myc蛋白和信使核糖核酸在细胞周期中的存在和合成。在细胞周期中，除了有丝分裂外，我们的c-myc基因在所有时间都处于相同的水平。该基因在G1期和G2期转录。对体内c-myc蛋白的分析表明，从头合成发生在G1和G2，该蛋白的半衰期为.apprx。两个阶段均需20-30分钟。当细胞重新启动细胞周期时，c-myc蛋白的水平在细胞群体中迅速增加，然后随着培养进入静止状态而下降。显然，c-myc的表达可以被快速调节，它在G0到G1的转变过程中被激活，但之后在细胞周期中表达。
The presence and synthesis of c-myc protein and mRNA in the cell cycle was studied. We c-myc mRNA is present at equivalent levels, at all times in the cell cycle with the possible exception of mitosis. This mRNA is transcribed in both G1 and G2 phases. An analysis of the c-myc protein in vivo shows that de novo synthesis occurs in G1 and G2 and the protein turns over with a half-life of .apprx. 20-30 min in both phases. The level of c-myc protein rapidly increases in cell populations when they re-initiate the cell cycle, thereafter decreasing as the culture reaches quiescence. Apparently, expression of c-myc can be rapidly modulated and that it is activated during the G0 to G1 transition, but is expressed thereafter in the cell cycle.