HISTOCHEMICAL AND ULTRASTRUCTURAL OBSERVATIONS ON BROWN DEGENERATION OF HUMAN INTERVERTEBRAL-DISK
HISTOCHEMICAL AND ULTRASTRUCTURAL OBSERVATIONS ON BROWN DEGENERATION OF HUMAN INTERVERTEBRAL-DISK
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DOI：
10.1002/jor.1100090111
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发表时间：
1991-01-01
影响因子：
2.8
通讯作者：
TERAHATA, N
中科院分区：
文献类型：
--
作者：
ISHII, T;TSUJI, H;TERAHATA, N
对前路椎间融合手术期间收集的 38 个新鲜人椎间盘进行组织化学和超微结构色素分析。宏观上，根据椎间盘髓核的颜色、纤维化和脆性将退变分为五个阶段。为了证明脂褐素颗粒，对样本进行了特殊的染色程序，包括卡博尔品红脂褐素染色、施莫尔反应和自发荧光。脂褐质颗粒从纤维环内层分布至髓核。在轻度或重度变性的情况下，此类颗粒较多，而在中度变性的情况下，发现的颗粒较少。然而，椎间盘宏观退变的阶段与脂褐素颗粒的发生率并不一定相关。通过超微结构观察，明确了椎间盘各成分的形态特征和脂褐素颗粒的超微结构。 “棕色变性”椎间盘的超微结构表现出位于基质中胶原纤维之间的无定形电子致密体显着增加。
Thirty-eight fresh human intervertebral discs collected during anterior interbody fusion surgery were histochemically and ultrastructurally analyzed for pigments. Macroscopically, five stages of degeneration were classified according to the color, fibrosis, and fragility fo the nucleus pulposus of the discs. In order to demonstrate lipofuscin granules, specimens were subjected to special staining procedures, including carbol fuchsin lipofuscin stain, the Schmorl's reaction, and autofluorescence. Lipofuscin granules were distributed from the inner layer of the annulus fibrosus to the nucleus pulposus. Such granules were numerous in cases of slight or severe degeneration, whereas fewer granules were found in cases of moderate degeneration. However, the stage of macroscopic degeneration of the intervertebral disc did not necessarily correlate with the incidence of lipofuscin granules. By ultrastructural observation, the morphological features of the components of the intervertebral disc and the ultrastructure of the lipofuscin granule were clarified. The ultrastructure of the "brown degeneration" disc exhibited markedly increased amorphous electron-dense bodies located among collagen fibrils in the matrix.