Inhibitory Smad proteins promote the differentiation of mouse embryonic stem cells into ependymal-like ciliated cells.
Inhibitory Smad proteins promote the differentiation of mouse embryonic stem cells into ependymal-like ciliated cells.
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DOI：
10.1016/j.bbrc.2010.08.099
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发表时间：
2010-10
影响因子：
3.1
通讯作者：
Y. Nishimura;A. Kurisaki;Mio Nakanishi;K. Ohnuma;Naoto Ninomiya;S. Komazaki;S. Ishiura;M. Asashima
中科院分区：
文献类型：
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作者：
Y. Nishimura;A. Kurisaki;Mio Nakanishi;K. Ohnuma;Naoto Ninomiya;S. Komazaki;S. Ishiura;M. Asashima
运动纤毛在维持体内平衡中起着至关重要的作用。纤毛的生物合成缺陷导致纤毛不动综合征，也称为原发性纤毛运动障碍（PCD），它与多种复杂疾病有关。在本研究中，我们发现Smad抑制蛋白Smad7和Smad6显著促进小鼠胚胎干细胞向纤毛细胞的分化。此外，这些Smad蛋白特异性诱导形态不同的musashi1阳性纤毛细胞。这些结果表明，Smad抑制蛋白不仅是纤毛细胞分化的重要调控因子，而且是小鼠胚胎干细胞分化过程中纤毛细胞亚型分化的重要调控因子。
Motile cilia play crucial roles in the maintenance of homeostasis in vivo. Defects in the biosynthesis of cilia cause immotile cilia syndrome, also known as primary ciliary dyskinesia (PCD), which is associated with a variety of complex diseases. In this study, we found that inhibitory Smad proteins, Smad7 and Smad6, significantly promoted the differentiation of mouse embryonic stem (ES) cells into ciliated cells. Moreover, these Smad proteins specifically induced morphologically distinct Musashi1-positive ciliated cells. These results suggest that inhibitory Smad proteins could be important regulators not only for the regulation of ciliated cell differentiation, but also for the subtype specification of ciliated cells during differentiation from mouse ES cells.