Arbuscular mycorrhizal colonization in field-collected terrestrial cordate gametophytes of pre-polypod leptosporangiate ferns (Osmundaceae, Gleicheniaceae, Plagiogyriaceae, Cyatheaceae)
Arbuscular mycorrhizal colonization in field-collected terrestrial cordate gametophytes of pre-polypod leptosporangiate ferns (Osmundaceae, Gleicheniaceae, Plagiogyriaceae, Cyatheaceae)
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DOI：
10.1007/s00572-015-0648-1
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发表时间：
2016-02-01
期刊：
影响因子：
3.9
通讯作者：
Imaichi, Ryoko
中科院分区：
文献类型：
--
作者：
Ogura-Tsujita, Yuki;Hirayama, Yumiko;Imaichi, Ryoko
为了确定蕨类植物配子体在不同类群中的菌根状态，我们在前水龙骨细孢蕨类蕨类植物的陆生心形配子体中研究了野生配子体的菌根定植，即一种紫萁科（Osmunda Banksiifolia）、两种皂角科（Diplopopterygium glaucum、 Dicranopteris Linearis），以及包括树蕨在内的四种桫椤目（桫椤科：Plagiogyria japonica、Plagiogyria euphlebia；桫椤科：Cyathea podophylla、Cyathea lepifera）。显微镜观察显示，所有物种中 58% 至 97% 的配子体都定殖有丛枝菌根 (AM) 真菌。在所有检查的配子体中，真菌定植仅限于多层中脉（垫）组织。利用真菌SSU rDNA序列进行分子鉴定表明，配子体中的AM真菌主要属于球菌科，但也包括Claroideoglomeraceae、Gigasporaceae、Acaulosporaceae和Archaeosporales等。这项研究为 AM 真菌在斜角藻科和桫椤科野生配子体中定殖提供了第一个证据。分类上不同的光合配子体同样被 AM 真菌定植，这表明与 AM 真菌的菌根关联可能广泛存在于陆生蕨类植物配子体中。
To determine the mycorrhizal status of pteridophyte gametophytes in diverse taxa, the mycorrhizal colonization of wild gametophytes was investigated in terrestrial cordate gametophytes of pre-polypod leptosporangiate ferns, i.e., one species of Osmundaceae (Osmunda banksiifolia), two species of Gleicheniaceae (Diplopterygium glaucum, Dicranopteris linearis), and four species of Cyatheales including tree ferns (Plagiogyriaceae: Plagiogyria japonica, Plagiogyria euphlebia; Cyatheaceae: Cyathea podophylla, Cyathea lepifera). Microscopic observations revealed that 58 to 97 % of gametophytes in all species were colonized with arbuscular mycorrhizal (AM) fungi. Fungal colonization was limited to the multilayered midrib (cushion) tissue in all gametophytes examined. Molecular identification using fungal SSU rDNA sequences indicated that the AM fungi in gametophytes primarily belonged to the Glomeraceae, but also included the Claroideoglomeraceae, Gigasporaceae, Acaulosporaceae, and Archaeosporales. This study provides the first evidence for AM fungal colonization of wild gametophytes in the Plagiogyriaceae and Cyatheaceae. Taxonomically divergent photosynthetic gametophytes are similarly colonized by AM fungi, suggesting that mycorrhizal associations with AM fungi could widely occur in terrestrial pteridophyte gametophytes.