Identification by peroxidase staining of monocytes in surface immunofluorescence tests.
Identification by peroxidase staining of monocytes in surface immunofluorescence tests.
复制标题
在表面免疫荧光测试中通过单核细胞的过氧化物酶染色进行鉴定。
DOI：
10.4049/jimmunol.113.5.1650
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发表时间：
1974
影响因子：
4.4
通讯作者：
Georges Flandrin
中科院分区：
文献类型：
--
作者：
Jean;Georges Flandrin
近年来，人们对人淋巴细胞上的表面免疫球蛋白（s.g ig2）进行了广泛的研究，主要是通过表面免疫荧光（IF）试验。这些研究导致了一些相互矛盾的结果（见1），大多数差异可能是由于方法原因造成的，因为s.g检测暴露于许多陷阱(2)。单核细胞是潜在错误的重要来源。它们通过Fc受体结合血清IgG，因此用共轭抗血清染色到γ-、κ-和λ-免疫球蛋白链。此外，一些单核细胞能够结合兔IgG作为试剂，并在IF测试中显示阳性，无论结合物的特异性是什么。为了避免由于单核细胞导致的错误解释，可以使用两种方法，要么使用不含单核细胞的细胞悬浮液，要么识别单核细胞。
Surface immunoglobulins (S.Ig)2 on human lymphocytes have been widely studied in the last few years, mainly with surface immunofluorescence (IF) tests. These studies have led to somewhat contradictory results (reviewed in 1) and most discrepancies are likely explained by methodological reasons since S.Ig detection is exposed to a number of pitfalls (2). Monocytes represent an important source of potential errors. They bind serum IgG by their Fc receptor and therefore stain with conjugated antisera to γ-, κ- and λ-immunoglobulin chains. In addition some monocytes are able to bind rabbit IgG used as reagents and show positivity in IF tests whatever the specificity of the conjugate is. Two approaches may be used in order to avoid erroneous interpretation due to monocytes, either the use of cell suspensions free of monocytes or the identification of monocytic cells.