Comparison analysis of different swabs and transport mediums suitable for SARS-CoV-2 testing following shortages
Comparison analysis of different swabs and transport mediums suitable for SARS-CoV-2 testing following shortages
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DOI：
10.1016/j.jviromet.2020.113947
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发表时间：
2020-11-01
影响因子：
3.1
通讯作者：
Strong, James E.
中科院分区：
文献类型：
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作者：
Garnett, Lauren;Bello, Alexander;Strong, James E.
2020年3月11日，世界卫生组织（WHO）将由SARS-CoV-2引起的COVID-19评估为大流行病。截至2020年6月1日，SARS-CoV-2已在全球范围内记录了超过600万例病例，导致超过37万人死亡（世界卫生组织，2020年）。新型冠状病毒（COVID-19）情况。http：//http：//covid19.who.int/）。因此，对检测的高需求导致了市售耗材的耗尽，包括鼻咽采样所需的推荐拭子和病毒转运培养基（VTM）。因此，必须探索未经验证的替代品的潜在用途，以解决全球检测用品短缺的问题。为了解决这个问题，我们评估了不同拭子和转运介质用于SARS-CoV-2分子检测的实用性。本研究比较了初级和三级医疗机构中常见的6种拭子（PurFlock Ultra、FLOQSwab、Puritan Pur-Wraps棉头施源器、Puritan聚酯头施源器、MedPro 6”棉头施源器和HOLOGIC Aptima）在检测SARS-CoV-2中的有效性。另外，SARS-CoV-2的分子检测从不同的转运培养基（DMEM、PBS、100%乙醇、0.9%生理盐水和VTM）完成，其在室温（RT）下保持长达3天。结果表明，用于SARS-CoV-2收集和检测的不同拭子和大多数转运培养基的病毒产量没有有意义的差异，表明如果供应耗尽，可以使用拭子和培养基替代品。
On March 11, 2020, the World Health Organization (WHO) assessed COVID-19, caused by SARS-CoV-2, as a pandemic. As of June 1, 2020, SARS-CoV-2 has had a documented effect of over 6 million cases world-wide, amounting to over 370,000 deaths (World Health Organization, 2020. Novel Coronavirus (COVID-19) Situation. http://http://covid19.who.int/). Consequently, the high demand for testing has resulted in a depletion of commercially available consumables, including the recommended swabs and viral transport media (VTM) required for nasopharyngeal sampling. Therefore, the potential use of unvalidated alternatives must be explored to address the global shortage of testing supplies. To tackle this issue, we evaluated the utility of different swabs and transport mediums for the molecular detection of SARS-CoV-2. This study compared the performance of six swabs commonly found in primary and tertiary health care settings (PurFlock Ultra, FLOQSwab, Puritan Pur-Wraps cotton tipped applicators, Puritan polyester tipped applicators, MedPro 6" cotton tipped applicators, and HOLOGIC Aptima) for their efficacy in testing for SARS-CoV-2. Separately, the molecular detection of SARS-CoV-2 was completed from different transport mediums (DMEM, PBS, 100 % ethanol, 0.9 % normal saline and VTM), which were kept up to three days at room temperature (RT). The results indicate that there is no meaningful difference in viral yield from different swabs and most transport mediums for the collection and detection of SARS-CoV-2, indicating swab and medium alternatives could be used if supplies run out.