Functional analysis of an archaebacterial voltage-dependent K+ channel
Functional analysis of an archaebacterial voltage-dependent K+ channel
复制标题
DOI：
10.1038/nature01473
复制
发表时间：
2003-03-13
期刊：
影响因子：
64.8
通讯作者：
MacKinnon, R
中科院分区：
文献类型：
--
作者：
Ruta, V;Jiang, YX;MacKinnon, R
所有活着的生物体都使用离子通道来调节离子在细胞膜上的运输。某些离子通道被归类为电压依赖型离子通道，因为它们具有电压敏感结构，可以根据细胞膜电压的变化诱导其毛孔打开。直到最近，电压依赖的K+、Ca~(2+)和Na~+通道被认为是真核细胞的一种独特的发育，适合于完成专门的电信号传递，如神经元。在这里，我们提出了一个电压依赖的K+(K-V)通道的功能特征，该通道来自海洋热喷口中的一种极端嗜热古细菌。该通道具有经典神经元K-V通道的所有功能属性。功能的保守反映了电压传感器的结构保守，这是通过与狼蛛毒素的特定的高亲和力相互作用来揭示的，这种作用进化到抑制真核细胞的K-V通道。
All living organisms use ion channels to regulate the transport of ions across cellular membranes'. Certain ion channels are classed as voltage-dependent because they have a voltage-sensing structure that induces their pores to open in response to changes in the cell membrane voltage. Until recently, the voltage-dependent K+, Ca2+ and Na+ channels were regarded as a unique development of eukaryotic cells, adapted to accomplish specialized electrical signalling, as exemplified in neurons. Here we present the functional characterization of a voltage-dependent K+ (K-V) channel from a hyperthermophilic archaebacterium from an oceanic thermal vent. This channel possesses all the functional attributes of classical neuronal K-V channels. The conservation of function reflects structural conservation in the voltage sensor as revealed by specific, high-affinity interactions with tarantula venom toxins, which evolved to inhibit eukaryotic K-V channels.