Dividing precursor cells of the embryonic cortical ventricular zone have morphological and molecular characteristics of radial glia
Dividing precursor cells of the embryonic cortical ventricular zone have morphological and molecular characteristics of radial glia
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DOI：
10.1523/jneurosci.22-08-03161.2002
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发表时间：
2002-04-15
影响因子：
5.3
通讯作者：
Kriegstein, AR
中科院分区：
文献类型：
--
作者：
Noctor, SC;Flint, AC;Kriegstein, AR
大脑皮层的胚胎脑室区（VZ）包含迁移神经元、放射状胶质细胞和大量产生新生神经元的循环祖细胞。一段时间以来，后两种细胞类别被认为在功能和解剖学上是不同的，但祖细胞类型的细胞解剖学仍然定义不清。最近的几篇报道通过证明放射状神经胶质细胞本身是神经元祖细胞，对放射状神经胶质细胞和前体细胞之间的区别提出了质疑（Malatesta et al.，2000; Hartfuss等人，2001; Miyata等人，2001; Noctor等人，2001年）。这一发现提出了放射状胶质细胞和VZ祖细胞群体可能是一种解剖学和功能性细胞类别的可能性。这种假说预测，在整个神经发生过程中，几乎所有有丝分裂活跃的VZ细胞和相当大比例的VZ细胞都是放射状胶质细胞。因此，我们使用各种解剖学，免疫组织化学和电生理技术来测试这些预测。我们的数据表明，大多数的VZ细胞，几乎所有的有丝分裂活跃的VZ细胞在神经发生，都有放射状胶质细胞形态和表达放射状胶质细胞标记。此外，细胞内染料填充的电生理特征的祖细胞在VZ表明，这些细胞具有放射状胶质细胞的形态。由于皮质VZ中的绝大多数循环细胞具有放射状胶质细胞的特征，因此放射状胶质前体细胞可能负责新生神经元的产生和子代神经元在发育中的皮质中的目的地的引导。
The embryonic ventricular zone (VZ) of the cerebral cortex contains migrating neurons, radial glial cells, and a large population of cycling progenitor cells that generate newborn neurons. The latter two cell classes have been assumed for some time to be distinct in both function and anatomy, but the cellular anatomy of the progenitor cell type has remained poorly defined. Several recent reports have raised doubts about the distinction between radial glial and precursor cells by demonstrating that radial glial cells are themselves neuronal progenitor cells (Malatesta et al., 2000; Hartfuss et al., 2001; Miyata et al., 2001; Noctor et al., 2001). This discovery raises the possibility that radial glia and the population of VZ progenitor cells may be one anatomical and functional cell class. Such a hypothesis predicts that throughout neurogenesis almost all mitotically active VZ cells and a substantial percentage of VZ cells overall are radial glia. We have therefore used various anatomical, immunohistochemical, and electrophysiological techniques to test these predictions. Our data demonstrate that the majority of VZ cells, and nearly all mitotically active VZ cells during neurogenesis, both have radial glial morphology and express radial glial markers. In addition, intracellular dye filling of electrophysiologically characterized progenitor cells in the VZ demonstrates that these cells have the morphology of radial glia. Because the vast majority cycling cells in the cortical VZ have characteristics of radial glia, the radial glial precursor cell may be responsible for both the production of newborn neurons and the guidance of daughter neurons to their destinations in the developing cortex.