ULTRASTRUCTURAL-LOCALIZATION OF ANTIGENIC SITES ON OSMIUM-FIXED TISSUES APPLYING THE PROTEIN A-GOLD TECHNIQUE
ULTRASTRUCTURAL-LOCALIZATION OF ANTIGENIC SITES ON OSMIUM-FIXED TISSUES APPLYING THE PROTEIN A-GOLD TECHNIQUE
复制标题
DOI：
10.1177/31.1.6187796
复制
发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
ZOLLINGER, M
中科院分区：
文献类型：
--
作者：
BENDAYAN, M;ZOLLINGER, M
蛋白质A-Au免疫细胞化学技术进行了修改，允许标记的细胞抗原位点的OS固定或postfixed组织。几种强氧化剂能够恢复蛋白质的抗原性，在组织切片的渗透。根据标记的精细结构保存和强度，偏高碘酸钠预处理得到最佳结果。胰腺分泌蛋白[来自大鼠]（和/或前蛋白）以及胰岛素（和/或胰岛素原）定位于保存完好的粗面内质网（rER）、高尔基体和相应胰腺细胞的分泌颗粒上;氨甲酰磷酸合成酶和过氧化氢酶分别位于肝线粒体和过氧化物酶体上。除了在使用Os固定的组织获得的标记中的更高分辨率之外，本发明的修改通过允许在常规EM处理的组织上进行标记而赋予蛋白质A-Au技术额外的优点。
The protein A-Au immunocytochemical technique was modified to allow labeling of cellular antigenic sites on Os-fixed or postfixed tissues. Several strong oxidizing agents were able to restore protein antigenicity on osmicated tissue thin sections. According to the fine structural preservation and intensities of labeling, pretreatment with sodium metaperiodate gave optimal results. Pancreatic secretory proteins [from rats] (and/or proproteins) as well as insulin (and/or proinsulin) were localized over perfectly preserved rough endoplasmic reticulum (rER), Golgi apparatus and secretory granules of the corresponding pancreatic cells; carbamyl phosphate synthetase and catalase were revealed over liver mitochondria and peroxisomes, respectively. In addition to the higher resolution in the labeling obtained using Os-fixed tissues, the present modification confers an additional advantage to the protein A-Au technique by allowing labeling on tissues processed for routine EM.