Adenyl cyclase of rat cerebral cortex. Activation of sodium fluoride and detergents.
Adenyl cyclase of rat cerebral cortex. Activation of sodium fluoride and detergents.
复制标题
大鼠大脑皮层的腺苷酸环化酶。
DOI：
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发表时间：
1971
影响因子：
4.8
通讯作者：
M. Moore
中科院分区：
文献类型：
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作者：
J. Perkins;M. Moore
氟化钠激活腺苷酸环化酶的速率依赖于温度和氟化钠浓度。通过稀释、膜结合酶的大量洗涤或透析，活化不容易逆转。活化在不存在添加的Mg++的情况下发生，但活化速率通过5 mm Mg++增加2倍。NaF活化的酶在40°和透析期间比天然酶更稳定。该酶也被Triton X-100激活，并在较小程度上被十二烷基硫酸钠激活。洗涤剂处理的酶的活性不受NaF刺激，但NaF活化的酶的活性可被Triton X-100增加。因此，NaF不引起腺苷酸环化酶活性的完全表达。冷冻和解冻的年轻（1至20天）大鼠的大脑皮层匀浆前的酶活性增加了2倍。腺苷酸环化酶以抑制状态存在于膜环境中。用洗涤剂处理或冷冻组织可能导致膜结构的非特异性变化，从而导致抑制作用降低和催化活性增加。
Abstract Sodium fluoride activates adenyl cyclase at a rate that is dependent on temperature and the concentration of NaF. The activation is not readily reversed by dilution, extensive washing of the membrane-bound enzyme, or dialysis. Activation occurs in the absence of added Mg++, but the rate of activation is increased 2-fold by 5 mm Mg++. The NaF-activated enzyme is more stable at 40° and during dialysis than the native enzyme. The enzyme also is activated by Triton X-100 and to a lesser extent by sodium dodecyl sulfate. The activity of the detergent-treated enzyme is not stimulated by NaF, but the activity of the NaF-activated enzyme can be increased by Triton X-100. Thus, NaF does not cause full expression of adenyl cyclase activity. Freezing and thawing of the cerebral cortex of young (1 to 20 days) rats prior to homogenization causes a 2-fold increase in enzyme activity. It was concluded that adenyl cyclase exists in its membranous environment in a state of inhibition. Treatment with detergents or freezing of the tissue could lead to nonspecific changes in membrane structure which cause reduction of the inhibition and increased catalytic activity.