Expression of the Arabidopsis DREB1A gene in transgenic chrysanthemum enhances tolerance to low temperature
Expression of the Arabidopsis DREB1A gene in transgenic chrysanthemum enhances tolerance to low temperature
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DOI：
10.1080/14620316.2006.11512162
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发表时间：
2006-11-01
影响因子：
1.9
通讯作者：
Gao, Jun-Ping
中科院分区：
文献类型：
--
作者：
Hong, Bo;Tong, Zheng;Gao, Jun-Ping
编码一种胁迫诱导转录因子的基因DREB1A，由花椰菜花叶病毒35S（CaMV）启动子或胁迫诱导的rd29A启动子驱动，通过农杆菌介导转化被转入菊花植株[菊（Dendranthema grandiflorum (Ramat.) Kitamura）品种‘秋彩’]中。通过聚合酶链式反应（PCR）和Southern杂交证实了目的基因DREB1A的整合。当暴露于2℃时，在幼龄的35S:DREB1A转基因植株系的根部，DREB1A的表达增强，在rd29A:DREB1A转基因植株系中被诱导表达。rd29A:DREB1A植株系叶片的电解质渗漏显著低于35S:DREB1A植株系。与对照植株相比，在冷胁迫处理开始时，转基因植株中超氧化物歧化酶活性和脯氨酸含量缓慢增加，并且在后期保持在较高水平，尤其是在rd29A:DREB1A转基因植株系中。rd29A:DREB1A植株系的幼龄植株能够耐受 -8℃达12小时，存活率为37.5%。在35S:DREB1A植株系或野生型植株中未观察到存活情况。这些结果表明，胁迫诱导的rd29A启动子和DREB1A基因的组合通过转基因方法增强了地被菊植株对冷胁迫的耐受性。
The gene DREB1A, encoding a stress-inducible transcription factor, driven by the cauliflower mosaic virus 35S (CaMV) promoter or by the stress-inducible rd29A promoter, was transferred into chrysanthemum plants [Dendranthema grandiflorum (Ramat.) Kitamura] cv. 'Fall Color' by Agrobacterium-mediated transformation. Integration of the target gene, DREB1A was confirmed by PCR and Southern blotting. When exposed to 2 degrees C, expression of DREB1A was enhanced in the roots of young transgenic 35S:DREB1A plant lines, and induced in transgenic rd29A:DREB1A lines. Electrolyte leakage in leaves of rd29A:DREB1A plant lines was significantly lower than in 35S:DREB1A lines. Compared to control plants, superoxide dismutase activities and proline contents increased slowly in transgenic plants at the start of the cold stress treatment and remained at high levels during later periods, especially in rd29A:DREB1A transgenic lines. Young plants of the rd29A:DREB1A line could tolerate -8 degrees C for 12 h, with a survival rate of 37.5 %. No survival was observed in 35S:DREB1A lines or in wild- type plants. These results indicate that a combination of the stress-inducible rd29A promoter and the DREB1A gene enhanced the tolerance of ground-cover chrysanthemum plants to cold stress through a transgenic approach.