DEAD-box RNA helicase DDX3X inhibits DENV replication via regulating type one interferon pathway
DEAD-box RNA helicase DDX3X inhibits DENV replication via regulating type one interferon pathway
复制标题
DEAD-box RNA 解旋酶 DDX3X 通过调节 1 型干扰素途径抑制 DENV 复制。
DOI：
10.1016/j.bbrc.2014.11.080
复制
发表时间：
2015-01-02
影响因子：
3.1
通讯作者：
Dai, Jianfeng
中科院分区：
文献类型：
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作者：
Li, Guanghao;Feng, Tingting;Dai, Jianfeng
登革热病毒（DENV）是一种蚊媒病毒，威胁着全球约25亿人。目前还没有针对DENV的疫苗。死亡盒RNA解旋酶（DDXs）参与病毒复制和宿主先天免疫应答。在本研究中，我们分析了40 DDX蛋白在DENV复制过程中的作用。其中，DDX 3X对DENV感染表现出抗病毒作用。与对照组相比，DDX 3X沉默细胞中的病毒复制显著增加。与对照组相比，DDX 3X沉默细胞中干扰素（IFN）-β的转录水平在DENV感染的早期阶段下降。DDX 3X可通过IRF 3和NF κ B B分支刺激DEN V感染细胞中IFN-β的转录。我们的数据表明，DDX 3X，一个成员的DEAD-box RNA解旋酶，是必要的IFN的生产，并能抑制登革病毒复制。(C)2014 Elsevier Inc. All rights reserved.
Dengue virus (DENV) is a mosquito-borne virus that threatens approximately 2.5 billion people worldwide. Vaccines against DENV are currently unavailable. DEAD-box RNA helicases (DDXs) have been reported to participate in viral replication and host innate immune response. In the present study, we analyzed the role of 40 DDX proteins during DENV replication. Among these proteins, DDX3X showed antiviral effect against DENV infection. Viral replication significantly increased in DDX3X-silenced cells compared with the controls. The interferon (IFN)-beta transcription level decreased during the early stage of DENV infection in DDX3X-silenced cells compared with that in the controls. DDX3X could stimulate IFN-beta transcription through the IRF3 and the NF kappa B branches in DEN V-infected cells. Our data imply that DDX3X, a member of DEAD-box RNA helicase, is necessary for IFN production and could inhibit DENV replication. (C) 2014 Elsevier Inc. All rights reserved.