SYNTHESIS AND ACCUMULATION OF DNA-LIKE RNA DURING EMBRYOGENESIS OF XENOPUS LAEVIS
SYNTHESIS AND ACCUMULATION OF DNA-LIKE RNA DURING EMBRYOGENESIS OF XENOPUS LAEVIS
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DOI：
10.1016/0022-2836(66)90119-7
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发表时间：
1966-01-01
影响因子：
5.6
通讯作者：
LITTNA, E
中科院分区：
文献类型：
--
作者：
BROWN, DD;LITTNA, E
建立了一种定量检测非洲爪哇胚胎发育过程中合成的类DNA RNA(DRNA)的技术。这项技术利用[P32]磷酸作为前体，并依赖于这样一个事实，即在相当长的一段时间内，核糖体RNA的合成低于dRNA的合成。通过测量酸溶池中RNA前体的[α]-磷酸盐比活度，并假设它们与dRNA的比活相同，将每个阶段dRNA中的放射性转换为dRNA含量。结果表明，成熟卵母细胞在排卵过程中合成约1~6m[mU]g的[32P]dRNA，约占卵细胞总RNA的0.02~0.1%。在发育过程中，胚胎中[P32]dRNA的含量相对于核糖体RNA有所增加，直到游泳胚胎中约2%的总RNA是[P32]dRNA。在胚胎发育早期，当dRNA增加相对核糖体时，胚胎中的[P32]dRNA含量每增加一倍，胚胎中的[P32]dRNA含量就会增加一倍。
A technique has been developed which quantitates the DNA-like RNA (dRNA) which is synthesized during embryogenesis of the amphibian Xenopus laevis. The technique utilizes [P32] phosphate as a precursor and relies on the fact that the synthesis of ribosomal RNA is low compared to that of dRNA for a considerable period of early embryogenesis. Radioactivity in dRNA at each stage has been converted into dRNA content by measuring the specific activities of the [alpha]-phosphates of RNA precursors in the acid-soluble pool and assuming that they are the same as the specific activity of dRNA. The results show that a mature oocyte synthesizes about 1 to 6 m [mu]g of [32P] dRNA during ovulation, an amount equal to about 0.02 to 0.1%, of the total RNA in the egg. During development, the content of [P32] dRNA in embryos increases relative to ribosomal RNA until about 2% of the total RNA in swimming embryos is [P32] dRNA. During early embryogenesis when dRNA increases relative ribosomes, the content of [P32] dRNA in the embryo approximately doubles with each doubling of the DNA content.