IDENTIFICATION AND CHARACTERIZATION OF A NOVEL YEAST GENE - THE YGP1 GENE-PRODUCT IS A HIGHLY GLYCOSYLATED SECRETED PROTEIN THAT IS SYNTHESIZED IN RESPONSE TO NUTRIENT LIMITATION
IDENTIFICATION AND CHARACTERIZATION OF A NOVEL YEAST GENE - THE YGP1 GENE-PRODUCT IS A HIGHLY GLYCOSYLATED SECRETED PROTEIN THAT IS SYNTHESIZED IN RESPONSE TO NUTRIENT LIMITATION
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DOI：
10.1128/mcb.14.4.2740
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发表时间：
1994-04-01
影响因子：
5.3
通讯作者：
KLIONSKY, DJ
中科院分区：
文献类型：
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作者：
DESTRUELLE, M;HOLZER, H;KLIONSKY, DJ
酵母的营养饥饿导致许多生理变化，伴随着进入静止期。其产物在应激适应中起作用的基因的表达以一种允许细胞感知和响应变化的环境条件的方式受到调节。我们已经鉴定出一种新的酵母基因YGP1，它与产孢特异性SPS100基因具有同源性。YGP1的表达受养分有效性的调控。在“呼吸发酵”（对数）生长过程中，该基因在基础水平上表达。当培养基中的葡萄糖浓度低于1%时，YGP1基因被抑制，基因产物gp37的合成水平高达基础水平的50倍。葡萄糖感知机制独立于SNF1途径，当细胞直接转移到低葡萄糖浓度时不起作用。YGP1的表达也响应氮和磷酸盐的消耗，表明对营养剥夺的一般反应。这些结果表明，YGP1基因产物可能参与了固定期之前的细胞适应，并且可能是监测与应激反应相关的早期事件的有用标记蛋白。
Nutrient starvation in the yeast Saccharomyces cerevisiae leads to a number of physiological changes that accompany entry into stationary phase. The expression of genes whose products play a role in stress adaptation is regulated in a manner that allows the cell to sense and respond to changing environmental conditions. We have identified a novel yeast gene, YGP1, that displays homology to the sporulation-specific SPS100 gene. The expression of YGP1 is regulated by nutrient availability. The gene is expressed at a basal level during ''respiro-fermentative'' (logarithmic) growth. When the glucose concentration in the medium falls below 1%, the YGP1 gene is derepressed and the gene product, gp37, is synthesized at levels up to 50-fold above the basal level. The glucose-sensing mechanism is independent of the SNF1 pathway and does not operate when cells are directly shifted to a low glucose concentration. The expression of YGP1 also responds to the depletion of nitrogen and phosphate, indicating a general response to nutrient deprivation. These results suggest that the YGP1 gene product may be involved in cellular adaptations prior to stationary phase and may be a useful marker protein for monitoring early events associated with the stress response.