Expression Profile of PTPIP51 in Mouse Brain
Expression Profile of PTPIP51 in Mouse Brain
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DOI：
10.1002/cne.22201
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发表时间：
2009-12-20
影响因子：
2.5
通讯作者：
Wimmer, M.
中科院分区：
文献类型：
--
作者：
Koch, P.;Viard, M.;Wimmer, M.
本研究证实了新型蛋白酪氨酸磷酸酶相互作用蛋白51 （PTPIP51）在哺乳动物脑组织中的表达。采用免疫组织化学、免疫印迹、RTPCR和原位杂交等方法，对成年小鼠全脑连续切片进行PTPIP51蛋白和mRNA的分析。Yu等人（2008）最近的研究表明，PTPIP51能够激活Raf-1，从而调节MAPK通路。Raf-1和14-3-3在神经系统疾病中的作用已得到证实。PTPIP51的表达仅限于梨状皮质及其与前联合、伏隔核、室旁核和视上核、脑垂体、上丘、面神经膝、脊髓三叉束、小脑下脚和小脑的连接。在小脑中，浦肯野细胞亚群及其树突呈强烈的PTPIP51阳性。此外，PTPIP51被发现与加压素及其转运蛋白神经physin 11共定位于神经内分泌核及其与神经垂体的连接。本文的数据表明，PTPIP51在神经元稳态、轴突生长和运输中发挥作用。中国生物医学工程学报，2009,17(5):591 - 595。(C) 2009 Wiley-Liss, Inc。
This study demonstrates the expression of the novel protein protein tyrosine phophatase-interacting protein 51 (PTPIP51) in mammalian brain tissue. Serial sections of the whole adult mouse brain were analyzed for PTPIP51 protein and mRNA by immunohistochemistry, immunoblotting, RTPCR, and in situ hybridization. Recent investigations by Yu et al. (2008) describe PTPIP51 as being capable of activating Raf-1, thereby modulating the MAPK pathway. The role of Raf-1, as well as of 14-3-3, in neurological disorders is well established. PTPIP51 expression was confined to neurons in the following structures: the piriform cortex and their connections to the anterior commissure, nucleus accumbens, paraventricular and supraoptical nuclei, neurohypophysis, superior colliculus, genu of facialis nerve, spinal trigeminal tract, inferior cerebellar peduncle, and cerebellum. In the cerebellum, a subpopulation of Purkinje cells and their dendrites was strongly PTPIP51 positive. Moreover, PTPIP51 was found to be colocalized with vasopressin and its transport protein neurophysin 11 in the neuroendocrine nuclei and their connections to the neurohypophysis. The data presented here suggest a role of PTPIP51 in neuronal homeostasis, axonal growth, and transport. J. Comp. Neurol. 517:892-905, 2009. (C) 2009 Wiley-Liss, Inc.