Discovery and initial characterization of YloC, a novel endoribonuclease in Bacillus subtilis.
Discovery and initial characterization of YloC, a novel endoribonuclease in Bacillus subtilis.
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DOI：
10.1261/rna.078962.121
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Bechhofer DH
中科院分区：
文献类型：
--
作者：
Ingle S;Chhabra S;Chen J;Lazarus MB;Luo X;Bechhofer DH
预计枯草芽孢杆菌基因组编码多种核糖核酸酶，包括4种已在一定程度上表征的3′外切核糖核酸酶。含有所有四种已知3′核糖核酸外切酶基因敲除的菌株是可行的，这表明一种或多种额外的RNA酶仍有待发现。将来自四重RNA酶突变株的蛋白质提取物分级分离，并跟踪RNA酶活性，从而鉴定由YloC蛋白催化的酶活性。YloC是一种核糖核酸内切酶，是细菌中广泛存在的高度保守的蛋白质“YicC家族”的成员。YloC是一种金属依赖性酶，其催化单链RNA的切割，优先在U残基处，并且以寡聚体形式存在，最可能是六聚体。因此，YloC与SARS-CoV Nsp 15内切核糖核酸酶具有一些共同特征。而YloC在B.虽然尚未确定是否存在枯草芽孢杆菌的小RNA，但发现YloC在评估小RNA RyhB的衰变的大肠杆菌体内测定中与YicC类似地起作用。因此，YloC可能在小RNA调节中起作用。
The Bacillus subtilis genome is predicted to encode numerous ribonucleases, including four 3′ exoribonucleases that have been characterized to some extent. A strain containing gene knockouts of all four known 3′ exoribonucleases is viable, suggesting that one or more additional RNases remain to be discovered. A protein extract from the quadruple RNase mutant strain was fractionated and RNase activity was followed, resulting in the identification of an enzyme activity catalyzed by the YloC protein. YloC is an endoribonuclease and is a member of the highly conserved “YicC family” of proteins that is widespread in bacteria. YloC is a metal-dependent enzyme that catalyzes the cleavage of single-stranded RNA, preferentially at U residues, and exists in an oligomeric form, most likely a hexamer. As such, YloC shares some characteristics with the SARS-CoV Nsp15 endoribonuclease. While the in vivo function of YloC in B. subtilis is yet to be determined, YloC was found to act similarly to YicC in an Escherichia coli in vivo assay that assesses decay of the small RNA, RyhB. Thus, YloC may play a role in small RNA regulation.
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影响因子：
14.8
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
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发表时间：
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作者：
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通讯作者：
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