Myzus persicae (green peach aphid) salivary components induce defence responses in Arabidopsis thaliana
Myzus persicae (green peach aphid) salivary components induce defence responses in Arabidopsis thaliana
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DOI：
10.1111/j.1365-3040.2009.02019.x
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发表时间：
2009-11-01
影响因子：
7.3
通讯作者：
Jander, Georg
中科院分区：
文献类型：
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作者：
De Vos, Martin;Jander, Georg
以拟南芥为食的桃蚜（绿桃蚜虫）在被侵染的叶片中引起防御反应，量化为蚜虫后代产量减少，但在植物的其他部分没有。同样，蚜虫唾液渗入拟南芥叶片只会引起蚜虫抗性的局部增强。对唾液防御诱导成分的进一步表征表明，拟南芥识别一个大小在3到10 kD之间的蛋白诱导子。利用具有良好特征的拟南芥突变体进行的遗传分析表明，唾液诱导的对桃蚜抗性不依赖于已知的防御信号通路，包括水杨酸、茉莉酸和乙烯。在蚜虫唾液浸润诱导的78个拟南芥基因中，已有52个基因被鉴定为蚜虫诱导的，但很少有基因对众所周知的植物防御信号分子水杨酸和茉莉酸有反应。定量PCR分析证实了唾液诱导基因的表达。尤其值得一提的是，桃蚜取食和用蚜虫唾液处理后，一组可能参与抗蚜硫代葡萄糖苷合成的o -甲基转移酶的表达显著上调。然而，这与4-甲氧基吲哚-3-基甲基硫代葡萄糖苷的产量增加无关，这表明蚜虫唾液成分触发拟南芥防御反应，独立于这种蚜虫威慑硫代葡萄糖苷。
Myzus persicae (green peach aphid) feeding on Arabidopsis thaliana induces a defence response, quantified as reduced aphid progeny production, in infested leaves but not in other parts of the plant. Similarly, infiltration of aphid saliva into Arabidopsis leaves causes only a local increase in aphid resistance. Further characterization of the defence-eliciting salivary components indicates that Arabidopsis recognizes a proteinaceous elicitor with a size between 3 and 10 kD. Genetic analysis using well-characterized Arabidopsis mutants shows that saliva-induced resistance against M. persicae is independent of the known defence signalling pathways involving salicylic acid, jasmonate and ethylene. Among 78 Arabidopsis genes that were induced by aphid saliva infiltration, 52 had been identified previously as aphid-induced, but few are responsive to the well-known plant defence signalling molecules salicylic acid and jasmonate. Quantitative PCR analyses confirm expression of saliva-induced genes. In particular, expression of a set of O-methyltransferases, which may be involved in the synthesis of aphid-repellent glucosinolates, was significantly up-regulated by both M. persicae feeding and treatment with aphid saliva. However, this did not correlate with increased production of 4-methoxyindol-3-ylmethylglucosinolate, suggesting that aphid salivary components trigger an Arabidopsis defence response that is independent of this aphid-deterrent glucosinolate.