Nanotopographic cell culture substrate: polymer-demixed nanotextured films under cell culture conditions.
Nanotopographic cell culture substrate: polymer-demixed nanotextured films under cell culture conditions.
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纳米形造影细胞培养基板：在细胞培养条件下聚合物二十种纳米刺的膜。
DOI：
10.1089/biores.2012.0255
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发表时间：
2012-10
影响因子：
--
通讯作者：
Donahue HJ
中科院分区：
文献类型：
--
作者：
Lim JY;Siedlecki CA;Donahue HJ
通过纳米尺度基底形貌修饰来调节物理细胞培养环境最近在再生医学中引起了极大的兴趣。许多研究已经利用聚合物分层技术来生产纳米纹理膜，并且表明细胞粘附、增殖和分化可以通过聚合物分层纳米形貌的形状和尺度来调节。然而，很少有人注意到的拓扑保真度的聚合物分层膜时，暴露于细胞培养条件。在这篇简短的文章中，使用两种聚合物分层系统来评估纤连蛋白（FN）细胞外基质蛋白吸附后和在37°C磷酸盐缓冲盐水中孵育后聚合物分层膜的地形变化。我们表明，FN吸附诱导非常小的变化（<2 nm）的聚苯乙烯/聚溴苯乙烯（PS/PBrS）-分层的纳米岛纹理，基本上不改变由聚合物分层给出的纳米形貌。此外，使用Mw=50×103的PLLA的聚（L-乳酸）/PS（PLLA/PS）-分层纳米岛形貌直到第24天未显示出显著降解。
Modulating physical cell culture environments via nanoscale substrate topographic modification has recently been of significant interest in regenerative medicine. Many studies have utilized a polymer-demixing technique to produce nanotextured films and showed that cellular adhesion, proliferation, and differentiation could be regulated by the shape and scale of the polymer-demixed nanotopographies. However, little attention has been paid to the topographic fidelity of the polymer-demixed films when exposed to cell culture conditions. In this brief article, two polymer-demixing systems were employed to assess topographic changes in polymer-demixed films after fibronectin (FN) extracellular matrix protein adsorption and after incubation in phosphate-buffered saline at 37°C. We showed that FN adsorption induced very small variations (<2 nm) to the polystyrene/polybromostyrene (PS/PBrS)-demixed nanoisland textures, not substantially altering the nanotopographies given by the polymer demixing. In addition, poly(L-lactic acid)/PS (PLLA/PS)-demixed nanoisland topographies using PLLA with Mw=50×103 did not show notable degradation up to day 24.