Transcriptional regulation of eater gene expression in Drosophila blood cells
Transcriptional regulation of eater gene expression in Drosophila blood cells
复制标题
DOI：
10.1002/dvg.20787
复制
发表时间：
2012-01-01
期刊：
影响因子：
1.5
通讯作者：
Schulz, Robert A.
中科院分区：
文献类型：
--
作者：
Kroeger, Paul T., Jr.;Tokusumi, Tsuyoshi;Schulz, Robert A.
Eater是一种介导果蝇吞噬作用的跨膜蛋白。在S2细胞中下调的基因的微阵列分析中鉴定了eater，其中Serpent已经被RNAi敲低。该基因被证明主要在胚胎发育后的浆细胞中表达。我们对控制eater表达的转录增强子进行了广泛的分析，发现了以下结果：通过抗P1抗体染色验证，该增强子再现了该基因的浆细胞表达模式，并且一个526个碱基对的DNA区域在淋巴腺和血淋巴浆细胞中具有活性。该DNA含有几个加塔元件，作为蛇的假定结合位点。这些加塔位点中的两个的定点诱变消除了淋巴腺和血淋巴浆细胞中的eater表达。这表明Serpent通过结合这些加塔位点调节eater表达，这通过凝胶位移分析证实。这些分析使我们能够使用eater-Gal 4迫使浆细胞分化为板层细胞。创世纪50：4149，2012。(C)2011 Wiley Periodicals，Inc.
Eater is a transmembrane protein that mediates phagocytosis in Drosophila. eater was identified in a microarray analysis of genes downregulated in S2 cells, in which Serpent had been knocked down by RNAi. The gene was shown to be expressed predominantly in plasmatocytes after embryonic development. We have extensively analyzed the transcriptional enhancer controlling eater expression with the following findings: the enhancer reproduces the plasmatocyte expression pattern of the gene as verified by anti-P1 antibody staining and a 526-basepair DNA region is active in lymph gland and hemolymph plasmatocytes. This DNA contains several GATA elements that serve as putative-binding sites for Serpent. Site-directed mutagenesis of two of these GATA sites abolishes eater expression in both lymph gland and hemolymph plasmatocytes. This suggests that Serpent regulates eater expression by binding these GATA sites, which was confirmed by gel shift analysis. These analyses allowed us to use eater-Gal4 to force plasmatocyte to lamellocyte differentiation. genesis 50:4149, 2012. (C) 2011 Wiley Periodicals, Inc.