Biomarkers of oxidative stress after controlled human exposure to ozone
Biomarkers of oxidative stress after controlled human exposure to ozone
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DOI：
10.1016/s0378-4274(02)00169-8
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发表时间：
2002-08-05
影响因子：
3.5
通讯作者：
Mutti, A
中科院分区：
文献类型：
--
作者：
Corradi, M;Alinovi, R;Mutti, A
本研究旨在评估受控短期暴露于臭氧（0，）是否会引起健康受试者呼出气冷凝液（EBC）和血液中肺部炎症和氧化应激生物标志物的变化。22名志愿者暴露于0.1 ppm的O-3 2小时，同时进行中度间歇运动。在暴露前、暴露后即刻和暴露后18 h采集EBC和血液。在EBC和血液中测量生物标志物的变化，而肺功能测试没有显著改变。EBC的变化，而不是在血液中，主要是由一个亚组的“易感”的个人承担野生基因型NAD（P）H：醌氧化还原酶（NQO 1）和谷胱甘肽-S-转移酶M1（GSTM 1）的无效基因型。因此，一个单一的2小时暴露于0.1 ppm的O-3诱导炎症和氧化应激的生物标志物的变化。多态性NQO 1和GSTM 1作为肺对O-3反应的修饰剂。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
This study was aimed at evaluating whether controlled short-term exposure to ozone (0,) induces changes in biomarkers of lung inflammation and oxidative stress in exhaled breath condensate (EBC) and blood of healthy subjects. Twenty-two volunteers were exposed to 0.1 ppm of O-3 for 2 h while performing moderate intermittent exercise. EBC and blood were collected before, immediately after and 18 h after exposure. Changes in biomarkers were measured both in EBC and blood, without significant alterations of lung function tests. Changes in EBC, but not in blood, were mainly accounted for by a subgroup of 'susceptible' individuals bearing the wild genotype for NAD(P)H:quinone oxidoreductase (NQO1) and the null genotype for glutathione-S-transferase M1 (GSTM1). Thus, a single 2-h exposure to 0.1 ppm of O-3 induces changes in biomarkers of inflammation and oxidative stress. Polymorphic NQO1 and GSTM1 act as modifier of the lung response to O-3. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.