Cytotoxicity for tumor cells of cationic proteins from human neutrophil granules.
Cytotoxicity for tumor cells of cationic proteins from human neutrophil granules.
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DOI：
10.1083/jcb.70.3.719
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发表时间：
1976-09
期刊：
影响因子：
--
通讯作者：
Klebanoff SJ
中科院分区：
文献类型：
--
作者：
Clark RA;Olsson I;Klebanoff SJ
从人多形核白细胞颗粒纯化的阳离子蛋白对哺乳动物肿瘤细胞具有细胞毒作用。这种作用与时间和浓度有关，被阴离子试剂肝素抑制，并通过预热阳离子蛋白质而增强。其他强碱性蛋白质（组蛋白、鱼精蛋白）也表现出细胞毒活性。从人类白细胞中分离出的髓过氧化物酶与 H2O2 和氯化物结合时具有细胞毒性。在这些条件下，髓过氧化物酶介导的系统的效力大于阳离子蛋白的效力。
Cationic proteins purified from human polymorphonuclear leukocyte granules exert a cytotoxic effect on mammalian tumor cells. This effect is time and concentration dependent, is inhibited by the anionic agent heparin, and is enhanced by preheating the cationic proteins. Other strongly basic proteins (histone, protamine) also exhibited cytotoxic activity. Myeloperoxidase isolated from human leukocytes is cytotoxic when combined with H2O2 and chloride. Under these conditions, the potency of the myeloperoxidase-mediated system is greater than that of the cationic proteins.