Analysis of cyclins A, B1, D1 and E in breast cancer in relation to tumour grade and other prognostic factors.
Analysis of cyclins A, B1, D1 and E in breast cancer in relation to tumour grade and other prognostic factors.
复制标题
DOI：
10.1186/1756-0500-2-140
复制
发表时间：
2009-07-17
影响因子：
1.8
通讯作者：
Hirsimäki P
中科院分区：
文献类型：
--
作者：
Boström P;Söderström M;Palokangas T;Vahlberg T;Collan Y;Carpen O;Hirsimäki P
细胞周期受细胞周期蛋白依赖性激酶(CDK)的激活促进，CDK受细胞周期蛋白的正向调节和CDK抑制剂的负向调节。肿瘤的增殖与细胞周期调节的改变有关。细胞周期蛋白A、B1、D1和E在乳腺癌中的联合变化与肿瘤分级和其他预后因素之间的关系尚不清楚。应用免疫组织化学方法检测53例乳腺癌组织中细胞周期蛋白A、B1、D_1、E、雌激素受体、孕激素受体、Ki-67、HER-2/neu和CK5/6的表达。用逆转录聚合酶链式反应(RT-PCR)分析12例标本中细胞周期蛋白的表达水平。细胞周期蛋白A、B1和E的表达呈正相关，而细胞周期蛋白D1的表达与上述三种细胞周期蛋白的表达无相关性。细胞周期蛋白A、B1和E与肿瘤分级、HER-2/neu和Ki-67相关。Cyclin E与激素受体呈负相关，与三阴性癌呈正相关。细胞周期蛋白D1与ER、PR及非基底细胞癌呈正相关。Cyclin A、B1和E的过度表达与肿瘤的分级、Ki-67和Her2/neu的表达有关。细胞周期蛋白D1的过度表达与受体状态和非基底细胞癌呈正相关，提示细胞周期蛋白D1的表达可能是预后良好的标志。对细胞周期蛋白的联合分析表明，细胞周期蛋白A、B和E的表达受相似的调控，而其他因素则调节细胞周期蛋白D1的表达。结果提示，细胞周期蛋白A、B1、D和E的联合免疫反应可能是判断乳腺癌预后的有用指标。
The cell cycle is promoted by activation of cyclin dependent kinases (Cdks), which are regulated positively by cyclins and negatively by Cdk inhibitors. Proliferation of carcinoma is associated with altered regulation of the cell cycle. Little is known on the combined alterations of cyclins A, B1, D1 and E in breast cancer in relation to the tumour grade and other prognostic factors. Immunohistochemical analysis of cyclins A, B1, D1 and E, estrogen receptor, progesterone receptor, Ki-67, Her-2/neu and CK5/6 was performed on 53 breast carcinomas. mRNA levels of the cyclins were analysed of 12 samples by RT-PCR. The expression of cyclins A, B1 and E correlated with each other, while cyclin D1 correlated with none of these cyclins. Cyclins A, B1 and E showed association with tumour grade, Her-2/neu and Ki-67. Cyclin E had a negative correlation with hormone receptors and a positive correlation with triple negative carcinomas. Cyclin D1 had a positive correlation with ER, PR and non-basal breast carcinomas. Cyclin A, B1 and E overexpression correlates to grade, Ki-67 and Her2/neu expression. Overexpression of cyclin D1 has a positive correlation with receptor status and non-basal carcinomas suggesting that cyclin D1 expression might be a marker of good prognosis. Combined analysis of cyclins indicates that cyclin A, B and E expression is similarly regulated, while other factors regulate cyclin D1 expression. The results suggest that the combined immunoreactivity of cyclins A, B1, D and E might be a useful prognostic factor in breast cancer.