The architecture of active zone material at the frog's neuromuscular junction
The architecture of active zone material at the frog's neuromuscular junction
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DOI：
10.1038/35054000
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发表时间：
2001-01-25
期刊：
影响因子：
64.8
通讯作者：
McMahan, UJ
中科院分区：
文献类型：
--
作者：
Harlow, ML;Ress, D;McMahan, UJ
神经系统突触的活性区材料位于停靠在突触前质膜上的突触囊泡旁边，锚定在膜上的钙通道。在这里，我们使用电子显微镜断层扫描来显示该紧凑型细胞器的结构成分的排列和关联，该模型突触是青蛙的神经肌肉连接。我们的发现表明，活动区域的材料有助于对接囊泡并锚定通道，并且其架构既提供了特定的空间关系，又提供了它们之间的结构联系。结构上的连锁可能包括蛋白质在突触传播过程中通过突触囊泡介导钙触发的神经递质的胞吐作用。
Active zone material at the nervous system's synapses is situated next to synaptic vesicles that are docked at the presynaptic plasma membrane, and calcium channels that are anchored in the membrane. Here we use electron microscope tomography to show the arrangement and associations of structural components of this compact organelle at a model synapse, the frog's neuromuscular junction. Our findings indicate that the active zone material helps to dock the vesicles and anchor the channels, and that its architecture provides both a particular spatial relationship and a structural linkage between them. The structural linkage may include proteins that mediate the calcium-triggered exocytosis of neurotransmitter by the synaptic vesicles during synaptic transmission.