Exosome-Mediated Delivery of the Neuroprotective Peptide PACAP38 Promotes Retinal Ganglion Cell Survival and Axon Regeneration in Rats With Traumatic Optic Neuropathy.
Exosome-Mediated Delivery of the Neuroprotective Peptide PACAP38 Promotes Retinal Ganglion Cell Survival and Axon Regeneration in Rats With Traumatic Optic Neuropathy.
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外泌体介导的神经保护肽 PACAP38 促进创伤性视神经病变大鼠的视网膜神经节细胞存活和轴突再生
DOI：
10.3389/fcell.2021.659783
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发表时间：
2021
影响因子：
5.5
通讯作者：
Yan H
中科院分区：
文献类型：
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作者：
Wang T;Li Y;Guo M;Dong X;Liao M;Du M;Wang X;Yin H;Yan H
外伤性视神经病变（TON）是指外伤引起的视神经损伤，导致视力部分或完全丧失。主要的治疗选择，如激素治疗和手术，疗效有限。腺苷酸环化酶激活多肽38（PACAP 38）是一种内源性神经保护肽，是一种很有前途的治疗药物。在这项研究中，我们使用大鼠视网膜神经节细胞（RGC）外泌体作为纳米囊泡，用于递送经由外泌体锚肽CP 05（EXOPACAP 38）加载的PACAP 38。EXOPACAP 38在体外和体内显示出比PACAP 38更高的摄取效率。结果显示，EXOPACAP 38显著增强大鼠TON模型中的RGC存活率和视网膜神经纤维层厚度。此外，EXOPACAP 38显著促进损伤后的轴突再生和视神经功能。这些研究结果表明，EXOPACAP 38可以作为一种治疗选择，并可能对TON患者具有治疗意义。
Traumatic optic neuropathy (TON) refers to optic nerve damage caused by trauma, leading to partial or complete loss of vision. The primary treatment options, such as hormonal therapy and surgery, have limited efficacy. Pituitary adenylate cyclase-activating polypeptide 38 (PACAP38), a functional endogenous neuroprotective peptide, has emerged as a promising therapeutic agent. In this study, we used rat retinal ganglion cell (RGC) exosomes as nanosized vesicles for the delivery of PACAP38 loaded via the exosomal anchor peptide CP05 (EXOPACAP38). EXOPACAP38 showed greater uptake efficiency in vitro and in vivo than PACAP38. The results showed that EXOPACAP38 significantly enhanced the RGC survival rate and retinal nerve fiber layer thickness in a rat TON model. Moreover, EXOPACAP38 significantly promoted axon regeneration and optic nerve function after injury. These findings indicate that EXOPACAP38 can be used as a treatment option and may have therapeutic implications for patients with TON.
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影响因子：
64.8
作者：
Lu Y;Brommer B;Tian X;Krishnan A;Meer M;Wang C;Vera DL;Zeng Q;Yu D;Bonkowski MS;Yang JH;Zhou S;Hoffmann EM;Karg MM;Schultz MB;Kane AE;Davidsohn N;Korobkina E;Chwalek K;Rajman LA;Church GM;Hochedlinger K;Gladyshev VN;Horvath S;Levine ME;Gregory-Ksander MS;Ksander BR;He Z;Sinclair DA
通讯作者：
Sinclair DA
影响因子：
11
作者：
Carta, A;Ferrigno, L;Carta, F
通讯作者：
Carta, F
影响因子：
13.7
作者：
Levin, LA;Beck, RW;Kraker, R
通讯作者：
Kraker, R
DOI：
10.1111/j.1749-6632.2010.05512.x
发表时间：
2010-01-01
期刊：
PHYLOGENETIC ASPECTS OF NEUROPEPTIDES: FROM INVERTEBRATES TO HUMANS
影响因子：
--
作者：
Atlasz, T.;Szabadfi, K.;Reglodi, D.
通讯作者：
Reglodi, D.
影响因子：
30.5
作者：
通讯作者：
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