In Vivo Formation of 8-Iso-Prostaglandin F2α and Platelet Activation in Diabetes Mellitus
In Vivo Formation of 8-Iso-Prostaglandin F2α and Platelet Activation in Diabetes Mellitus
复制标题
DOI：
10.1161/01.cir.99.2.224
复制
发表时间：
1999-01
期刊：
影响因子：
37.8
通讯作者：
G. Davı̀;G. Ciabattoni;A. Consoli;A. Mezzetti;A. Falco;S. Santarone;E. Pennese;E. Vitacolonna;T. Bucciarelli;F. Costantini;F. Capani;C. Patrono
中科院分区：
文献类型：
--
作者：
G. Davı̀;G. Ciabattoni;A. Consoli;A. Mezzetti;A. Falco;S. Santarone;E. Pennese;E. Vitacolonna;T. Bucciarelli;F. Costantini;F. Capani;C. Patrono
背景-糖尿病（DM）与脂质过氧化作用增强和血小板持续活化有关。我们检验了以下假设：在DM中，F2-异前列烷8-异前列腺素（PG）F2α（花生四烯酸过氧化的生物活性产物）的体内形成增强，并有助于血小板活化。方法和结果：从85名糖尿病患者和85名年龄和性别匹配的健康受试者中采集尿液样本，用于测量免疫反应性8-iso-PGF 2 α和11-脱氢血栓烷B2（TXM），这是体内血小板活化的指标。62例为非胰岛素依赖型（NID）DM，23例为胰岛素依赖型（ID）DM。使用维生素E补充剂、代谢控制和环氧合酶抑制剂来研究这种情况下8-iso-PGF 2 α的形成机制。NIDDM患者的尿8-iso-PGF 2 α排泄量（419±208 pg/mg肌酐;范围160 - 1014）显著高于（P=0.0001）年龄匹配的对照受试者（208±92; 41 - 433）。尿8-异丙...
Background—Diabetes mellitus (DM) is associated with enhanced lipid peroxidation and persistent platelet activation. We tested the hypothesis that the in vivo formation of the F2-isoprostane 8-iso-prostaglandin (PG)F2α, a bioactive product of arachidonic acid peroxidation, is enhanced in DM and contributes to platelet activation. Methods and Results—Urine samples were obtained from 85 diabetic patients and 85 age- and sex-matched healthy subjects for measurement of immunoreactive 8-iso-PGF2α and 11-dehydro-thromboxane B2 (TXM), an in vivo index of platelet activation. Sixty-two had non–insulin-dependent (NID)DM, and 23 had insulin-dependent (ID) DM. Vitamin E supplementation, metabolic control, and cyclooxygenase inhibitors were used to investigate the mechanisms of formation of 8-iso-PGF2α in this setting. Urinary 8-iso-PGF2α excretion was significantly higher (P=0.0001) in NIDDM patients (419±208 pg/mg creatinine; range 160 to 1014) than in age-matched control subjects (208±92; 41 to 433). Urinary 8-iso...