Biopsy of a biopsy: validation of immunoprofiling in gastric cancer biopsy tissue microarrays
Biopsy of a biopsy: validation of immunoprofiling in gastric cancer biopsy tissue microarrays
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DOI：
10.1046/j.1365-2559.2003.01556.x
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发表时间：
2003-01-01
期刊：
影响因子：
6.4
通讯作者：
Leader, M
中科院分区：
文献类型：
--
作者：
Gulmann, C;Butler, D;Leader, M
目的：组织微阵列提供了一种有效的方法来检查一个单一的载玻片上的大量肿瘤病例。然而，一个主要的问题是肿瘤的异质性。此外，组织微阵列在活检材料中的使用尚未探索。本研究的目的是评估的可能性和有效性，每例在内镜下胃癌活检的免疫phenotype.Methods和结果：38例进行了研究与p53，CD 44 v6和血管内皮生长因子的免疫组化染色的三个0.6毫米核心。将完整组织切片与三核心组织微阵列进行比较。36例包含3个肿瘤核心，1例包含2个肿瘤核心，1例仅包含1个存活肿瘤核心，被排除。在免疫组织化学切片上，三个单独病例的另外三个核心丢失。全切片与组织微阵列的Kappa值在0.77和0.94之间。p53免疫组化染色（解释为+或-）产生了最好的结果，只有1/37的错配，而CD 44 v6（强度和程度分级）显示3/37的错配。活检组织芯中的组织深度较小，因此所有芯必须排列成与受体蜡块表面齐平，以最大限度地增加可用切片的数量。与第一节相比，超过30个额外的4-妈妈节之前，第一个情况下（与一个核心左）已被排除，80节之前，一半的组织核心losed.Conclusions：它是不切实际的阵列超过120-150核心每块。每例三芯组织芯片用于研究活检材料是可行和有效的。
Aims: Tissue microarrays offer an efficient way of examining a large number of tumour cases on a single glass slide. A major concern, however, is tumour heterogeneity. Also, the use of tissue microarrays in biopsy material is unexplored. The purpose of the present study was to assess the possibility and validity of arraying three 0.6-mm cores per case in endoscopic gastric cancer biopsies for immunophenotyping.Methods and results: Thirty-eight cases were studied with immunohistochemical staining for p53, CD44v6 and vascular endothelial growth factor. Full tissue sections were compared with triple core-tissue microarrays. Thirty-six cases contained three cores with tumour, one case contained two cores with tumour and one case contained only a single core with viable tumour and was excluded. Three further cores had been lost from three separate cases on the sections for immunohistochemistry. kappa values for whole-sections versus tissue microarrays ranged between 0.77 and 0.94. p53 immunohistochemical staining (interpretation as + or -) yielded the best result with only 1/37 mismatches, whereas CD44v6 (graded both for intensity and extent) showed 3/37 mismatches. The small depth of tissue in cores from biopsies necessitates all cores being arrayed flush with the face of the recipient wax block for maximizing the number of sections available. Compared with the first section over 30 additional 4-mum sections were available before the first case (with one core left) had to be excluded and 80 sections before half the tissue cores were lost.Conclusions: It is impracticable to array more than 120-150 cores per block. Tissue microarray with three cores per case is feasible and valid for studying biopsy material.