Bacteria co-culture adhesion on different texturized zirconia surfaces.
Bacteria co-culture adhesion on different texturized zirconia surfaces.
复制标题
细菌在不同纹理氧化锆表面上的共培养粘附。
DOI：
10.1016/j.jmbbm.2021.104786
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发表时间：
2021
影响因子：
3.9
通讯作者：
F. Silva
中科院分区：
文献类型：
--
作者：
T. Dantas;J. Padrão;Mariana Rodrigues da Silva;P. Pinto;S. Madeira;P. Vaz;A. Zille;F. Silva
氧化锆被认为是不同应用的有前途的解决方案，特别是在牙科种植体研究领域。它已被证明可以成功克服常用钛种植体的重要局限性。微生物（特别是细菌）对种植体的粘附可决定牙种植体的成功或失败，因为细菌在种植体周围骨上的积累可迅速演变成牙周炎。然而，细菌粘附在不同的氧化锆架构仍然是相当未知的。因此，大肠杆菌，金黄色葡萄球菌和铜绿假单胞菌的粘附氧化锆表面与不同的fineneficians进行了评估，并比较钛表面。S. aureusandP.还使用共培养物评价了产气荚膜梭菌，因为这些细菌由于它们在慢性伤口感染中的常见存在而声名狼借。结果表明，不同的细菌物种具有不同的属性，这影响了它们对不同粗糙度和结构的粘附倾向。大肠杆菌对氧化锆表面的粘附能力较高（7.15 × 106 CFU/mL），而S. aureusandP.铜绿假单胞菌的粘附率高于钛对照组（分别为1.07 × 105 CFU/mL和8.43 × 106 CFU/mL）。此外，共培养对细菌的粘附行为表现出显着差异。尽管没有表现出特别好的细菌粘附行为，但具有微通道的氧化锆表面有望改善种植体周围的血管化，并最终增强骨整合，因此是牙科种植体的有前途的解决方案。
Zirconia is becoming reckoned as a promising solution for different applications, in particular those within the dental implant investigation field. It has been proved to successfully overcome important limitations of the commonly used titanium implants. The adhesion of microorganisms to the implants, in particular of bacteria, may govern the success or the failure of a dental implant, as the accumulation of bacteria on the peri-implant bone may rapidly evolve into periodontitis. However, bacterial adhesion on different zirconia architectures is still considerably unknown. Therefore, the adhesion ofEscherichia coli,Staphylococcus aureusandPseudomonasaeruginosato zirconia surfaces with different finishings was evaluated and compared to a titanium surface. The adhesion interaction betweenS. aureusandP. aeruginosawas also evaluated using a co-culture since these bacteria are infamous due to their common presence in chronic wound infections. Results showed that different bacterium species possess different properties which influence their propensity to adhere to different roughness levels and architectures.E. colirevealed a higher propensity to adhere to zirconia channelled surfaces (7.15 × 106CFU/mL), whereasS. aureusandP. aeruginosaadhered more to the titanium control group (1.07 × 105CFU/mL and 8.43 × 106CFU/mL, respectively). Moreover, the co-culture denoted significant differences on the adhesion behaviour of bacteria. Despite not having shown an especially better behaviour regarding bacterial adhesion, zirconia surfaces with micro-channels are expected to improve the vascularization around the implants and ultimately enhance osseointegration, thus being a promising solution for dental implants.