Effects of root-end filling materials and eugenol on mitochondrial dehydrogenase activity and cytotoxicity to human periodontal ligament fibroblasts
Effects of root-end filling materials and eugenol on mitochondrial dehydrogenase activity and cytotoxicity to human periodontal ligament fibroblasts
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DOI：
10.1002/jbm.b.30107
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发表时间：
2004-11-15
影响因子：
3.4
通讯作者：
Jeng, JH
中科院分区：
文献类型：
--
作者：
Lin, CP;Chen, YJ;Jeng, JH
各种根端填充材料已被用来防止根管病原体进入根尖周区域。使用新型 Transwell 培养系统，比较了五种根端充填材料的人牙周膜 (PDL) 成纤维细胞的细胞毒性、细胞凋亡和线粒体脱氢酶 (MDH) 活性。暴露于 IRM（一种基于 ZnO 丁香酚的中间修复材料）、2-乙氧基苯甲酸水泥 (Super EBA) 和汞合金 3 天可抑制 PDL 成纤维细胞的 MDH 活性，如 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物 (MTT) 减少 97%、95% 和分别为 51%。暴露 5 天后，发现汞齐和玻璃离聚物水门汀 (GIC) 对 MTT 还原的明显抑制，分别为 73% 和 46%。三氧化二矿物质聚集体 (MTA) 对 MDH 活性几乎没有影响。台盼蓝染料排除技术表明，IRM 和 Super EBA 对 PDL 成纤维细胞具有细胞毒性。 GIC 和汞合金表现出轻微的细胞毒性。 IRM、GIC 和汞合金进一步诱导 PDL 细胞凋亡，流式细胞仪直方图中存在亚 G0/G1 DNA 含量表明这一点。暴露于 IRM 和 Super EBA 24 小时后，MDH 活性分别提高至对照的 156% 和 117%。丁香酚是 Super EBA 和 IRM 中的酚类成分，在浓度为 0.5 和 1 mM 时，还可将 PDL 成纤维细胞的 MDH 活性提高 45% 和 51%。然而，当浓度高于 0.5 mM 时，丁子香酚会减少活 PDL 成纤维细胞的数量。这些结果表明 MTA 是一种生物相容性根端充填材料，其次是自固化 Fuji II GIC 和汞合金。 IRM和Super EBA成分诱导显着的细胞毒性并短暂刺激MDH活性，这可能是由于它们的丁子香酚含量和诱导细胞适应性反应。 (C) 2004 年 Wiley 期刊公司。
Various root-end filling materials have been used to prevent the entry of root-canal pathogens into periapical regions. Five root-end filling materials were compared regarding the cytotoxicity, apoptosis, and mitochondrial dehydrogenase (MDH) activities of human periodontal ligament (PDL) fibroblasts, with the use of a novel transwell culture system. Exposure to IRM (a ZnO eugenol-based intermediate restorative material), a 2-ethoxybenzoic acid cement (Super EBA), and amalgam for 3 days inhibited the MDH activity of PDL fibroblasts as indicated by decrease in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) reduction by 97%, 95%, and 51%, respectively. Evident suppression of MTT reduction by amalgam and glass ionomer cement (GIC) was noted after 5 days of exposure, with 73% and 46% of inhibition, respectively. Mineral trioxide aggregates (MTA) showed little effect on MDH activity. IRM and Super EBA were cytotoxic to PDL fibroblasts as indicated by a trypan blue dye exclusion technique. GIC and amalgam showed mild cytotoxicity. IRM, GIC, and amalgam further induced apoptosis of PDL cells, as revealed by the presence of sub-G0/G1 DNA content in flow cytometric histogram. Twenty-four-hour exposure to IRM and Super EBA elevated the MDH activities to 156% and 117%, correspondingly, of that of control. Eugenol, a phenolic ingredient in Super EBA and IRM, also increases MDH activity of PDL fibroblasts by 45% and 51%, at concentrations of 0.5 and 1 mM. However, at concentrations higher than 0.5 mM, eugenol decreased the number of viable PDL fibroblasts. These results suggest that MTA is a biocompatible root-end filling material, followed by self-curing Fuji II GIC and amalgam. IRM and Super EBA ingredients induced marked cytotoxicity and transiently stimulate MDH activities, which is possibly due to their content of eugenol and induction of cellular adaptive response. (C) 2004 Wiley Periodicals, Inc.