Expression of Collagen (Types I, III, and V), HSP47, MMP-2, and TIMP-1 in Retrobulbar Adipose Tissue of Patients with Thyroid-Associated Orbitopathy
Expression of Collagen (Types I, III, and V), HSP47, MMP-2, and TIMP-1 in Retrobulbar Adipose Tissue of Patients with Thyroid-Associated Orbitopathy
复制标题
甲状腺相关眼眶病患者球后脂肪组织中胶原蛋白（I、III 和 V 型）、HSP47、MMP-2 和 TIMP-1 的表达
DOI：
10.1155/2020/4929634
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发表时间：
2020-04-23
影响因子：
1.9
通讯作者：
Luo, Xin
中科院分区：
文献类型：
--
作者：
Luo, Hongyang;Liu, Taixiang;Luo, Xin
目标。本研究旨在探讨甲状腺相关眼眶病(TAO)患者球后脂肪组织中I、III、V型胶原、热休克蛋白47(HSP47)、基质金属蛋白酶-2(MMP2)及其组织抑制因子-1(TIMP-1)的表达。材料和方法收集自2019年5月至2019年9月4例TAO眼眶减压术患者和4例眼外伤致眼球萎缩的眼球摘除患者的球后脂肪组织。Masson染色分析各标本胶原表达及组织学纤维化程度的差异。免疫印迹法检测I型、III型、V型胶原、HSP47、MMP2、TIMP-1的蛋白表达。Western blotting数据用SPSS17.0版软件进行统计分析，采用独立t检验。结果。Masson染色结果显示，TAO组胶原纤维表达明显高于对照组，纤维弥漫且分布不规则。TAO组I型、III型、V型胶原、HSP47、MMP2、TIMP-1的表达水平显著高于对照组(P
Objective. This study aimed to investigate the expression of collagen (types I, III, and V), heat shock protein 47 (HSP47), matrix metalloproteinase-2 (MMP-2), and tissue inhibitors of metalloproteinase-1 (TIMP-1) in the retrobulbar adipose tissues of patients with thyroid-associated orbitopathy (TAO). Materials and Methods. The retrobulbar adipose tissues were collected from 4 TAO patients undergoing orbital decompression and 4 ocular enucleation patients with atrophic eyeball caused by ocular trauma between May 2019 and September 2019. Masson staining was performed to analyze the differences in collagen expression and degree of histologic fibrosis in each sample. The protein expressions of collagen (types I, III, and V), HSP47, MMP-2, and TIMP-1 were determined by western blotting. The data of western blotting were analyzed using SPSS version 17.0, with independent t-tests. Results. The results of Masson staining showed that the expression of collagen fibers in the TAO group was significantly higher than that in the control group, and the fibers were diffuse and irregular in distribution. The expression level of collagen (types I, III, and V), HSP47, MMP-2, and TIMP-1 in the TAO group were significantly higher than that in the control group (P