Development and characterizations of a miniature capillary electrophoresis mass spectrometry system.
Development and characterizations of a miniature capillary electrophoresis mass spectrometry system.
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DOI：
10.1021/ac504868w
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发表时间：
2015-01
影响因子：
7.4
通讯作者：
Muyi He;Zhen Xue;Yinna Zhang;Zejian Huang;Xiang Fang;Feng Qu;Z. Ouyang;W. Xu
中科院分区：
文献类型：
--
作者：
Muyi He;Zhen Xue;Yinna Zhang;Zejian Huang;Xiang Fang;Feng Qu;Z. Ouyang;W. Xu
本文研制了一种小型毛细管电泳质谱联用系统。100%电驱动的小型化CE装置与微型MS仪器集成，其具有用于与大气压电离源耦合的不连续大气压接口（DAPI）。研制了一种具有鞘液接口的纳米电喷雾离子源，用于耦合微型毛细管电泳和质谱系统。系统的表征和分析性能的优化已经完成。已经证明了在纳米ESI源中分析同量异位肽和避免电荷竞争效应。
A miniature capillary electrophoresis mass spectrometry (CE/MS) system has been developed in this work. A 100% electrical driven miniaturized CE device was integrated with a miniature MS instrument, which has a discontinuous atmospheric pressure interface (DAPI) for coupling with atmospheric pressure ionization sources. A nanoelectrospray ionization (nano-ESI) source was developed with a sheath liquid interface for coupling the miniature CE and the MS system. A systematic characterization and optimization of the analytical performance have been done. The analysis of isobaric peptides and avoiding charge competition effects in nano-ESI sources have been demonstrated.