High-performance genetic analysis on microfabricated capillary array electrophoresis plastic chips fabricated by injection molding
High-performance genetic analysis on microfabricated capillary array electrophoresis plastic chips fabricated by injection molding
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DOI：
10.1021/ac0485031
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发表时间：
2005-04-01
影响因子：
7.4
通讯作者：
Baba, Y
中科院分区：
文献类型：
--
作者：
Dang, FQ;Tabata, O;Baba, Y
We have developed a novel technique for mass production of microfabricated capillary array electrophoresis (mu-CAE) plastic chips for high-speed, high-throughput genetic analysis. The mu-CAE chips, containing 10 individual separation channels of 50-mu m width, 50-mu m depth, and a 100-mu m lane-to-lane spacing at the detection region and a sacrificial channel network, were fabricated on a poly(methyl methacrylate) substrate by injection molding and then bonded manually using a pressure-sensitive sealing tape within several seconds at room temperature. The conditions for injection molding and bonding were carefully characterized to yield mu-CAE chips with well-defined channel and injection structures. A CCD camera equipped with an image intensifier was used to monitor simultaneously the separation in a 10-channel array with laser-induced fluorescence detection. High-performance electrophoretic separations of phi X174 HaeIII DNA restriction fragments and PCR products related to the human beta-globin gene and SP-B gene (the surfactant protein B) have been demonstrated on mu-CAE plastic chips using a methylcellulose sieving matrix in individual channels. The current work demonstrated greatly simplified the fabrication process as well as a detection scheme for mu-CAE chips and will bring the low-cost mass production and application of mu-CAE plastic chips for genetic analysis.
