STEREOCILIARY BUNDLE MORPHOLOGY IN ORGANOTYPIC CULTURES OF THE MOUSE COCHLEA
STEREOCILIARY BUNDLE MORPHOLOGY IN ORGANOTYPIC CULTURES OF THE MOUSE COCHLEA
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DOI：
10.1016/0378-5955(89)90131-7
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发表时间：
1989-03-01
期刊：
影响因子：
2.8
通讯作者：
RUSSELL, IJ
中科院分区：
文献类型：
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作者：
FURNESS, DN;RICHARDSON, GP;RUSSELL, IJ
新生小鼠耳蜗的器官型培养物具有由3-5排外毛细胞和单排内毛细胞组成的毛细胞带。外毛细胞静纤毛束从顶端向基端逐渐分化。未分化的顶束具有类似微绒毛的短静纤毛的盘状阵列。部分分化的束是半球形的，具有组织不良的密集的静纤毛行，其高度在动纤毛的方向上逐渐增加。更分化的束保持半球形，具有许多微绒毛样静纤毛，但沿着最靠近动纤毛的一侧具有高度组织化的静纤毛行，并且行之间具有明确的高度增量。高度分化的基生维管束通常呈“V”或“W”形，尽管有些几乎是多边形。基束有4-5排规则的静纤毛，每排的静纤毛高度有明显的梯度，很少有微绒毛状的静纤毛。只有在更分化的束中才能观察到交叉连接，其中静纤毛的行是规则的，并且在它们之间具有显著的高度增量。链接显示了各种各样的形式和方向，以前没有观察到在其他准备。许多静纤毛的上部区域投射出辐条状连接阵列，其他静纤毛似乎具有明显的尖端到侧边连接，尽管具有各种方向。在出生后早期的耳蜗在体内，但不是在成年小鼠的耳蜗中观察到类似的各种交联，这表明，这种品种可能是一个短暂的功能，静纤毛束的发展。在离体条件下，内毛细胞静纤毛束常被发育中的盖膜覆盖。内毛细胞束及其交联的形态变化与外毛细胞束相似。
Organotypic cultures of the neonatal mouse cochlea have a band of hair cells consisting of 3-5 rows of outer hair cells and a single row of inner hair cells. The outer hair cell stereocilliary bundles show progressive differentiation from the apical to the basal ends of the band. Undifferentiated apical bundles have a disk-like array of short stereocilia resembling microvilli. Partially differentiated bundles are hemispherical with poorly organized rows of thickly clustered stereocilia, which gradually increase in height in the direction of the kinocilium. More differentiated bundles remain hemispherical with many microvilli-like stereocilia, but have highly organized rows of stereocilia along the side nearest to the kinocilium, and well-defined height increments between the rows. Highly-differentiated, basal bundles usually have a ''V'' or ''W'' shape, although some can be almost polygonal. The basal bundles have 4-5 regular rows of stereocilia with a well-defined gradient in height across the rows, and very few microvilli-like stereocilia. Cross-links are only consistently observed in more differentiated bundles, where the rows of stereocilia are regular and have significant height increments across them. The links show a wide variety of forms and orientations not previously observed in other preparations. Spoke-like arrays of links project from the upper regions of many stereocilia and other stereocilia appear to bear distinct tip-to-side links, although with a variety of orientations. A similar variety of cross-links is observed in early postnatal cochleae in vivo, but not in the cochleae of adult mice, indicating that this variety may be a transient feature of stereociliary bundle development. In vitro, inner hair cell stereociliary bundles are often covered by overlying material from the developing tectorial membrane. The variations in morphology if inner hair cell bundles and their cross-links are similar to those of the outer hair cell bundles.