Identification and characterization of regulatory elements in the phosphoenolpyruvate carboxykinase gene PCK1 of Saccharomyces cerevisiae
Identification and characterization of regulatory elements in the phosphoenolpyruvate carboxykinase gene PCK1 of Saccharomyces cerevisiae
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DOI：
10.1007/bf00288610
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发表时间：
1995-02
期刊：
影响因子：
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通讯作者：
M. Proft;D. Grzesitza
中科院分区：
文献类型：
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作者：
M. Proft;D. Grzesitza
Phosphoenolpyruvate carboxykinase is a key enzyme in gluconeogenesis. The expression of thePCK1gene inSaccharomyces cerevisiaeis strictly regulated and dependent on the carbon source provided. Two upstream activation sites (UAS1PCK1and UAS2PCK1) and one upstream repression site (URSPCK1) were localized by detailed deletion analysis. The efficacy of these three promoter elements when separated from each other was confirmed by investigations using heterologous promoter test plasmids. Activation mediated by UAS1PCK1or UAS2PCK1did not occur in the presence of glucose, indicating that these elements are essential for glucose derepression. The repressing effect caused by URSPCK1was much stronger in glucose-grown cells than in ethanol-grown cells.
