A novel bacteriophage cocktail reduces and disperses Pseudomonas aeruginosa biofilms under static and flow conditions.
A novel bacteriophage cocktail reduces and disperses Pseudomonas aeruginosa biofilms under static and flow conditions.
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DOI：
10.1111/1751-7915.12316
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发表时间：
2016-01
影响因子：
5.7
通讯作者：
Jenkins AT
中科院分区：
文献类型：
--
作者：
Alves DR;Perez-Esteban P;Kot W;Bean JE;Arnot T;Hansen LH;Enright MC;Jenkins AT
铜绿假单胞菌是一种机会性人类病原体，可形成高度稳定的群落——生物膜，有助于感染的建立和维持。生物膜状态和内在/获得性细菌耐药机制导致对抗生素的耐药性/耐受性，这在疟原虫中经常观察到。 铜绿假单胞菌分离株。在这里，我们描述了六种新型裂解性噬菌体的分离和表征：感染细菌的病毒，它们共同有效地感染和杀死多种噬菌体。 铜绿假单胞菌临床分离株。这些噬菌体被用来配制具有消除疟原虫潜力的鸡尾酒。 铜绿假单胞菌 PAO1 浮游培养物。研究了两种生物膜模型，一种是静态的，一种是动态的，并评估了噬菌体混合物减少和分散生物膜生物量的能力。对于静态模型，与噬菌体悬浮液（MOI 10）接触4小时后，超过95%的生物膜生物量被消除。在流动生物膜模型中，观察到噬菌体的活动速率较慢，但添加噬菌体混合物 48 小时后，生物膜分散，与对照相比，大多数细胞被消除（> 4 个对数）。这种鸡尾酒具有开发作为控制疟原虫的治疗方法的潜力。 铜绿假单胞菌感染，主要以生物膜为中心。
P seudomonas aeruginosa is an opportunistic human pathogen that forms highly stable communities – biofilms, which contribute to the establishment and maintenance of infections. The biofilm state and intrinsic/acquired bacterial resistance mechanisms contribute to resistance/tolerance to antibiotics that is frequently observed in P . aeruginosa isolates. Here we describe the isolation and characterization of six novel lytic bacteriophages: viruses that infect bacteria, which together efficiently infect and kill a wide range of P . aeruginosa clinical isolates. The phages were used to formulate a cocktail with the potential to eliminate P . aeruginosa PAO1 planktonic cultures. Two biofilm models were studied, one static and one dynamic, and the phage cocktail was assessed for its ability to reduce and disperse the biofilm biomass. For the static model, after 4 h of contact with the phage suspension (MOI 10) more than 95% of biofilm biomass was eliminated. In the flow biofilm model, a slower rate of activity by the phage was observed, but 48 h after addition of the phage cocktail the biofilm was dispersed, with most cells eliminated (> 4 logs) comparing with the control. This cocktail has the potential for development as a therapeutic to control P . aeruginosa infections, which are predominantly biofilm centred.