Induction and differentiation of adipose-derived stem cells from human buccal fat pads into salivary gland cells.
Induction and differentiation of adipose-derived stem cells from human buccal fat pads into salivary gland cells.
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DOI：
10.1007/s13577-016-0132-z
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发表时间：
2016-07
期刊：
影响因子：
4.3
通讯作者：
Mataga I
中科院分区：
文献类型：
--
作者：
Kawakami M;Ishikawa H;Tanaka A;Mataga I
由于衰老或疾病导致的唾液腺萎缩或功能减退导致唾液分泌不足，导致口干、咀嚼/吞咽功能恶化以及口腔卫生状况不佳，从而影响患者的生活质量。目前临床实践中唾液腺萎缩或功能减退的治疗侧重于使用药物和人工唾液缓解症状；因此，仍然需要开发新的疗法。为了研究潜在的新治疗靶点，我们通过将从颊脂肪垫分离的人脂肪源性干细胞（hBFP-ASC）与人唾液腺源性成纤维细胞（hSG-纤维）共培养来诱导唾液腺细胞的分化。我们检查了它们移植和组织新生的潜力。培养hBFP-ASC和hSG-纤维后，将分化的细胞移植到SCID小鼠的下颌下腺中，并测定它们在组织中的分化程度。我们还使用三维 (3D) 培养系统检查了它们功能性组织重建的潜力。共培养的细胞表达唾液腺相关标记，并在体内移植后产生新的组织。此外，细胞在 3D 培养系统中重建腺体结构。总之，hSG-纤维与 hBFP-ASC 的共培养导致成功分化为唾液腺细胞，可以移植以产生新组织。
Atrophy or hypofunction of the salivary gland because of aging or disease leads to hyposalivation that affects patient quality of life by causing dry mouth, deterioration of mastication/deglutition, and poor oral hygiene status. Current therapy for atrophy or hypofunction of the salivary gland in clinical practice focuses on symptom relief using drugs and artificial saliva; therefore, there is still a need to develop new therapies. To investigate potential novel therapeutic targets, we induced the differentiation of salivary gland cells by co-culturing human adipose-derived stem cells isolated from buccal fat pads (hBFP-ASCs) with human salivary-gland-derived fibroblasts (hSG-fibros). We examined their potential for transplantation and tissue neogenesis. Following the culture of hBFP-ASCs and hSG-fibros, differentiated cells were transplanted into the submandibular glands of SCID mice, and their degree of differentiation in tissues was determined. We also examined their potential for functional tissue reconstitution using a three-dimensional (3D) culture system. Co-cultured cells expressed salivary-glandrelated markers and generated new tissues following transplantation in vivo. Moreover, cell reconstituted glandular structures in the 3D culture system. In conclusion, coculture of hSG-fibros with hBFP-ASCs led to successful differentiation into salivary gland cells that could be transplanted to generate new tissues.