Identification and high yield purification of insulin-like growth factors (nonsuppressible insulin-like activities and somatomedins) from human plasma by use of endogenous binding proteins.
Identification and high yield purification of insulin-like growth factors (nonsuppressible insulin-like activities and somatomedins) from human plasma by use of endogenous binding proteins.
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使用内源结合蛋白从人血浆中鉴定和高产纯化胰岛素样生长因子（不可抑制的胰岛素样活性和生长调节素）。
DOI：
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发表时间：
1979
影响因子：
5.8
通讯作者：
G. Baumann
中科院分区：
文献类型：
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作者：
B. Ginsberg;C. Kahn;J. Roth;K. Megyesi;G. Baumann
The insulin-like growth factors (IGF) of human serum, somatomedins A and C, and nonsuppressible insulin-like activities (NSILA) I and II are available in only a few laboratories and in very limited quantity. In the present study we have identified three peptides with insulin-like activity and have partially purified two of them from human plasma by a high yield method that utilizes the natural affinity of these peptides for their binding protein(s). Human plasma was ultrafiltered at neutral pH and the insulin-like growth factors, which were associated with high molecular weight binding protein(s), were retained, while insulin and other low molecular weight polypeptides were eliminated. The retentate was acidified to dissociate the peptides from their binding protein(s) and the ultrafiltration was repeated. The dissociated peptides were collected in the ultrafiltrate. Further purification was obtained on gel filtration on Sephadex G-75. Two peaks of activity were observed with apparent molecular weights be...
