Is phosphorylation the main physiological action of myosin light chain kinase?
Is phosphorylation the main physiological action of myosin light chain kinase?
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磷酸化是肌球蛋白轻链激酶的主要生理作用吗？
DOI：
10.1139/y94-198
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发表时间：
1994
影响因子：
2.1
通讯作者：
H. Kuwayama
中科院分区：
文献类型：
--
作者：
S. Ebashi;H. Kuwayama
牛胃155 kDa组分具有较强的肌动球蛋白(AM)激活活性和相对较弱的肌球蛋白轻链激酶(MLCK)活性，它与肌动蛋白细丝有很强的亲和力，其N末端的肌动蛋白结合部位仅有80个氨基酸残基。这种亲和力可能在AM激活中起关键作用。有些试剂优先取消AM激活效应或MLCK活性。总之，就体外系统而言，155 kDa组分的MLCK在激活AM系统中并不起基础作用。讨论了该组分激活AM的可能机制。
The 155-kDa component of bovine stomach, which exhibits a strong actomyosin (AM) activating activity and a relatively weak myosin light chain kinase (MLCK) activity, has a strong affinity for the actin filament and the actin-binding site is confined to an 80 amino acid residue on its N-terminal side. This affinity may play a crucial role in AM activation. Some reagents preferentially abolish either the AM-activating effect or MLCK activity. In conclusion, MLCK of the 155-kDa component does not play a fundamental role in activating the AM system as far as the in vitro system is concerned. The possible mechanism of AM activation by the component is discussed.
肌球蛋白轻链激酶抗体对化学透化平滑肌收缩性和肌球蛋白磷酸化的影响。
影响因子：
20.1
作者：
DeLanerolle,P;Strauss,JD;Felsen,R;Doerman,GE;Paul,RJ
通讯作者：
Paul,RJ