Galectin-3 binds lactosaminylated lipooligosaccharides from Neisseria gonorrhoeae and is selectively expressed by mucosal epithelial cells that are infected
Galectin-3 binds lactosaminylated lipooligosaccharides from Neisseria gonorrhoeae and is selectively expressed by mucosal epithelial cells that are infected
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DOI：
10.1046/j.1462-5822.2002.00219.x
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发表时间：
2002-10-01
影响因子：
3.4
通讯作者：
Griffiss, JM
中科院分区：
文献类型：
--
作者：
John, CM;Jarvis, GA;Griffiss, JM
半乳糖凝集素是β -半乳糖苷结合蛋白的一个家族，由于β -半乳糖苷碳水化合物在细菌膜糖脂低聚糖（LOS）和脂多糖中很常见，因此被认为是细菌的宿主受体。我们研究了半乳糖凝集素-3与淋球菌LOS的相互作用，这些LOS可以产生乳糖基（Lc(2)或Lac）、副叶糖基(nLc(4)；LNnT;乳酸-n -新四糖)、神经节糖基（IV3 GalNAcnLc(4)）和新乳酸己糖基（nLc(6)、内酯或己糖基）低聚糖。除了神经节苷基以外，所有LOS都终止于-半乳糖苷。半乳糖凝集素-3对nLc(6) LOS具有最高的亲和力，nLc(6) LOS是由一种对男性尿道具有高度传染性的菌株产生的，但也能结合nLc(4) LOS和Lac LOS。与乳糖或N -乙酰乳胺相比，乳酸-N -新四糖四糖是一种更有效的半乳糖凝集素-3与LOS结合的抑制剂。半乳糖凝集素-3对淋球菌的相对亲和力反映了其对纯化LOS的亲和力。Western blot分析显示，半乳糖凝集素-3在人子宫内膜腺癌和可被淋球菌侵袭的前列腺上皮细胞中表达。人输卵管上皮免疫组化结果显示，非纤毛细胞局部表达半乳糖凝集素-3，是淋球菌侵袭的特异性细胞。我们得出结论，由于它的位置和对淋球菌的亲和力，半凝集素-3可能在淋球菌感染中发挥作用。
Galectins are a family of beta-galactoside binding proteins that have been proposed as host receptors for bacteria because beta-galactoside carbohydrates are common in bacterial membrane glycolipid lipooligosaccharides (LOS) and lipopolysaccharides. We investigated the interaction of galectin-3 with gonococcal LOS that make lactosyl (Lc(2) or Lac), paraglobosyl (nLc(4) ; LNnT; lacto-N -neotetraose), gangliosyl (IV3 GalNAcnLc(4) ), and neolactohexaosyl (nLc(6) , lactonorhexaosyl) oligosaccharides. All but gangliosyl LOS terminate in beta-galactoside. Galectin-3 had the highest affinity for the nLc(6) LOS, which is made by a strain that is highly infectious for the male urethra, but also bound nLc(4) LOS and to a Lac LOS. The lacto-N -neotetraose tetrasaccharide was a more potent inhibitor of galectin-3 binding to LOS than either lactose or N -acetyllactosamine. The relative affinity of galectin-3 for gonococci mirrored its affinity for purified LOS. Western blot analysis revealed expression of galectin-3 by human endometrial adenocarcinoma and prostatic epithelial cells that can be invaded by gonococci. Immunohistochemistry of human fallopian tube epithelium showed localized expression of galectin-3 by non-ciliated cells, the specific cell gonococci invade in this tissue. We conclude that because of its location and affinity for gonococcal LOS galectin-3 could play a role in gonococcal infection.