Langerhans cells are essential components of the angiogenic niche during murine skin repair.
Langerhans cells are essential components of the angiogenic niche during murine skin repair.
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DOI：
10.1016/j.devcel.2022.11.012
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发表时间：
2022-12
影响因子：
11.8
通讯作者：
Renee R. Wasko;Kate Bridges;Rebecca Pannone;Ikjot Sidhu;Yue Xing;S. Naik;K. Miller-Jensen;V. Horsley
中科院分区：
文献类型：
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作者：
Renee R. Wasko;Kate Bridges;Rebecca Pannone;Ikjot Sidhu;Yue Xing;S. Naik;K. Miller-Jensen;V. Horsley
血管生成是指在发育、损伤修复和肿瘤发生过程中，由原有血管生成的新血管向组织输送氧气、免疫细胞和营养物质。血管生成缺陷发生在心血管疾病和炎症性疾病，以及慢性、不愈合的伤口，但治疗选择有限。在这里，我们通过分析小鼠皮肤伤口愈合的单细胞RNA测序，提供了早期血管生成生态位的地图。我们的数据表明朗格汉斯细胞（LCs），吞噬细胞，皮肤驻留免疫细胞，在皮肤修复过程中驱动血管生成。使用谱系驱动的报告器，三维（3D）显微镜和小鼠遗传学，我们发现LCs位于小鼠皮肤伤口的内皮细胞前沿，并且是修复过程中血管生成所必需的。这些数据为控制血管生成以治疗疾病和慢性伤口提供了额外的未来途径，并扩展了LCs在抗原呈递和T细胞免疫中的典型作用之外的功能。
Angiogenesis, the growth of new blood vessels from pre-existing vessels, occurs during development, injury repair, and tumorigenesis to deliver oxygen, immune cells, and nutrients to tissues. Defects in angiogenesis occur in cardiovascular and inflammatory diseases, and chronic, non-healing wounds, yet treatment options are limited. Here, we provide a map of the early angiogenic niche by analyzing single-cell RNA sequencing of mouse skin wound healing. Our data implicate Langerhans cells (LCs), phagocytic, skin-resident immune cells, in driving angiogenesis during skin repair. Using lineage-driven reportersw, three-dimensional (3D) microscopy, and mouse genetics, we show that LCs are situated at the endothelial cell leading edge in mouse skin wounds and are necessary for angiogenesis during repair. These data provide additional future avenues for the control of angiogenesis to treat disease and chronic wounds and extend the function of LCs beyond their canonical role in antigen presentation and T cell immunity.