LENS MEMBRANES .2. ISOLATION AND CHARACTERIZATION OF MAIN INTRINSIC POLYPEPTIDE (MIP) OF BOVINE LENS FIBER MEMBRANES
LENS MEMBRANES .2. ISOLATION AND CHARACTERIZATION OF MAIN INTRINSIC POLYPEPTIDE (MIP) OF BOVINE LENS FIBER MEMBRANES
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DOI：
10.1016/0014-4835(76)90135-4
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发表时间：
1976-01-01
影响因子：
3.4
通讯作者：
STOLS, ALH
中科院分区：
文献类型：
--
作者：
BROEKHUYSE, RM;KUHLMANN, ED;STOLS, ALH
对牛晶状体纤维膜的主要内在多肽（MIP, MW 26,500）进行了分离和表征。用氯仿-甲醇法从尿素处理过的晶状体膜中提取。提取总膜蛋白的23%，占总MIP的1/3。从缓冲洗膜中提取MIP失败表明它与基质蛋白结合。气相色谱分析结果表明，该产品不含糖残基。从氨基酸组成来看，MIP具有较低的极性，不同于β -氨基酸。Bp结晶蛋白链和其他可溶性结晶蛋白。免疫化学结果表明，MIP不与任何晶体蛋白发生交叉反应，结合其他数据表明，MIP是一种典型的膜成分。
The main intrinsic polypeptide (MIP, MW 26,500) of bovine lens fiber membranes was isolated and characterized. It was extracted from urea-treated lens membranes by chloroform-methanol. The extraction recovered 23% of the total membrane protein which is 1/3 of total MIP. Failure to extract MIP from buffer-washed membranes suggested that it was bound to matrix protein. Gas chromatography showed that it contained no sugar residues. On the basis of its amino acid composition, MIP had a low polarity and was different from the .beta.Bp crystallin chain and from other soluble crystallins. Immunochemically, it did not cross-react with any of the crystallins, which together with the other data indicated that MIP is a typical membrane component.