A combination of metabolomics and metallomics studies of urine and serum from hypercholesterolaemic rats after berberine injection
A combination of metabolomics and metallomics studies of urine and serum from hypercholesterolaemic rats after berberine injection
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DOI：
10.1007/s00216-012-5923-9
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发表时间：
2012-05-01
影响因子：
4.3
通讯作者：
Li, Sam Fong Yau
中科院分区：
文献类型：
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作者：
Liu, Feng;Gan, Pei Pei;Li, Sam Fong Yau
小檗碱长期以来在中国和印度被用作治疗肠道感染的药物，近年来在西方国家被发现，现在被用于治疗尿路感染、糖尿病和肥胖等疾病。为了更深入地研究小檗碱的作用，本研究采用高胆固醇血症大鼠模型，开发了代谢组学和金属组学相结合的方法，其中包括使用质子核磁共振分析大鼠尿液以实现代谢指纹图谱和电感耦合等离子体质谱分析大鼠血清以实现金属组学指纹图谱。结果表明，注射小檗碱后，高胆固醇血症大鼠的主要代谢过程，如克雷布斯循环、胆固醇代谢和渗透压调节受到干扰。此外，金属组学研究中揭示的V、Mn、Na和K等元素的变化可能有助于寻找高胆固醇血症的新生物标志物。我们得出的结论是，小檗碱治疗的高胆固醇血症大鼠的代谢组学和金属组学特征均不同于对照组，并且所选代谢物和元素可能可以用作潜在的生物标志物，以了解小檗碱对动物模型生化过程的影响。这种多重分析方法可能会为阐明异生素的影响和药物功效研究提供一个信息丰富的平台。
Berberine, long used as a remedy in China and India for intestinal infections, has been discovered in recent years in western countries and is now being used to treat ailments ranging from urinary tract infections to diabetes and obesity. In order to study the effect of berberine more deeply, a combined metabolomic and metallomic approach was developed in this study using the hypercholesterolaemic rat model, which involved the use of proton nuclear magnetic resonance for the analysis of rat urine to achieve metabolic fingerprinting and inductively coupled plasma mass spectrometry for the analysis of rat blood serum to achieve metallomic fingerprinting. The results obtained indicated that major metabolic processes like Krebs cycle, cholesterol metabolism and osmoregulation in hypercholesterolaemic rats are perturbed upon berberine injection. In addition, the changes of some elements, such as V, Mn, Na and K, revealed in the metallomic study may contribute to the search of new biomarkers for hypercholesterolaemic disease. We concluded that both the metabolomic and metallomic profiles of berberine-treated hypercholesterolaemic rats were different from those of the control group and that the selected metabolites and elements could probably be applied as potential biomarkers for the understanding of the effect of berberine on biochemical process in the animal model. Such a multi-analytical approach will potentially provide an information-rich platform for the elucidation of effects of xenobiotics and drug efficacy studies.