Peripheral Blood Th1 and Th2 Profile in Patients with Moderate Asthma: Effect of Inhaled Corticosteroid
Peripheral Blood Th1 and Th2 Profile in Patients with Moderate Asthma: Effect of Inhaled Corticosteroid
复制标题
中度哮喘患者外周血 Th1 和 Th2 谱：吸入皮质类固醇的作用
作者：
Y. Nagano;M. Kondo;J. Tamaoki;K. Isono;A. Nagai
用12-肉豆酸13-乙酸佛波酯和离子霉素刺激健康人及哮喘患者外周血，对细胞进行抗cd4抗体染色、通透化、抗ifn -γ和抗il -4抗体染色，流式细胞术分析。与健康受试者相比，哮喘患者IL-4(+) IFN-γ(-) CD4细胞（Th2细胞）和IFN-γ(+) IL-4(-) CD4细胞（Th1细胞）的百分比均较高。Th2细胞百分比与血清IgE水平相关。吸入皮质类固醇治疗后，Th2细胞在第24周下降，但在第4周没有下降。因此，可能需要长期吸入类固醇治疗来改善淋巴细胞炎症。
The peripheral blood from healthy subjects and asthma patients was stimulated with phorbol 12-myristate 13-acetate and ionomycin, and the cells were stained with anti-CD4 antibody, permeabilized, stained with anti-IFN-γ and anti-IL-4 antibodies, and analyzed by flow cytometry. Compared with healthy subjects, asthma patients showed a greater percentage of both IL-4(+) IFN-γ (–) CD4 cells (Th2 cells) and IFN-γ(+) IL-4(–) CD4 cells (Th1 cells). The percentage of Th2 cells was correlated with serum IgE level. After treatment with inhaled corticosteroid, Th2 cells decreased at week 24, but not week 4. Long-term therapy with inhaled steroid may thus be required for improvement in lymphocytic inflammation.
影响因子：
20.3
作者：
PICKER, LJ;SINGH, MK;MAINO, VC
通讯作者：
MAINO, VC