The Genomic Context for the Evolution and Transmission of Community-Associated Staphylococcus aureus ST59 Through the Food Chain
The Genomic Context for the Evolution and Transmission of Community-Associated Staphylococcus aureus ST59 Through the Food Chain
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DOI：
10.3389/fmicb.2020.00422
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发表时间：
2020-03-17
影响因子：
5.2
通讯作者：
Wu Qingping
中科院分区：
文献类型：
--
作者：
Pang Rui;Wu Shi;Wu Qingping
序列类型 59 (ST59) 是亚洲社区获得性耐甲氧西林金黄色葡萄球菌 (CA-MRSA) 的主要克隆谱系。尽管 ST59 在中国的临床意义日益增强，但它的进化和地理扩张却相对不受关注。此前的研究表明，ST59是中国食品相关MRSA的主要克隆。本研究比较了来自食物链和感染病例的 87 个克隆复合体 (CC) 59 金黄色葡萄球菌分离株的基因组，以重建 ST59 的分子进化和地理传播。因此，鉴定了 ST59 的三个主要亚进化枝，这些亚进化枝与分离源或位置无关。系统发育分析估计，中国大陆的ST59在1974年左右与最常见的最近祖先分离，大多数跨国传播病例发生在1987年至2000年之间。值得注意的是，最近观察到两起通过食物链进行跨国传播的事件，这些事件中的分离株在相对较短的时间间隔内发生了分化。这些分离株在核心基因组、辅助基因和抗生素耐药性模式方面也表现出高度相似性。这些发现为了解 ST59 在中国的潜在传播途径提供了宝贵的见解，并表明需要进行强有力的食物链监测以防止这种病原体的传播。
Sequence type 59 (ST59) is a predominant clonal lineage of community-acquired, methicillin-resistant Staphylococcus aureus (CA-MRSA) in Asia. Despite its increasing clinical relevance in China, the evolution and geographic expansion of ST59 has been relatively uncared for. Previous study has shown that ST59 was the predominant clone in food-related MRSA in China. This study compared the genomes of 87 clonal complex (CC) 59 S. aureus isolates sourced from food chain and infection cases to reconstruct the molecular evolution and geographical spread of ST59. Accordingly, three major sub-clades of ST59 were identified and these did not correlate with isolation source or location. Phylogenetic analysis estimated that ST59 in mainland China diverged from a most common recent ancestor around 1974, and most of the cases of cross-country transmission occurred between 1987 and 2000. Notably, two recent events of cross-country transmission through the food chain were observed, the isolates from these events diverged within relatively short time intervals. These isolates also showed high similarity in terms of their core genome, accessory genes, and antibiotic resistance patterns. These findings provide a valuable insight into the potential route of ST59 expansion in China and indicate a need for robust food chain surveillance to prevent the spread of this pathogen.