Mus musculus Barrier-To-Autointegration Factor 2 (Banf2) is Not Essential for Spermatogenesis or Fertility
Mus musculus Barrier-To-Autointegration Factor 2 (Banf2) is Not Essential for Spermatogenesis or Fertility
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小家鼠自体整合屏障因子 2 (Banf2) 对于精子发生或生育力不是必需的。
DOI：
10.1159/000513850
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发表时间：
2021-05-05
影响因子：
1.7
通讯作者：
Zheng, Ying
中科院分区：
文献类型：
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作者：
Niu, Chang-Min;Xia, Meng-Meng;Zheng, Ying
自整合屏障因子（BAF）广泛表达于人体组织中，在染色质组构、核膜组装、性腺发育和胚胎干细胞自我更新中起重要作用。BAF的完全丧失已被证明会导致胚胎死亡和性腺缺陷。BAF蛋白，即自整合屏障因子2（BANF 2），在人类和小鼠中均表现出睾丸主导的表达模式。与BAF不同，它可能导致孤立的男性不育。因此，我们使用CRISPR/Cas9系统产生Banf 2敲除小鼠，以进一步研究其在精子发生中的功能。出乎意料的是，敲除小鼠的睾丸、附睾、卵巢和其他组织的组织结构没有显示出任何可检测到的异常，并且表现出正常的生育能力，这表明Banf 2对小鼠精子发生和生育能力不是必需的。
The barrier-to-autointegration factor (BAF) is widely expressed in most human tissues and plays a critical role in chromatin organization, nuclear envelope assembly, gonadal development, and embryonic stem cell self-renewal. Complete loss of BAF has been shown to lead to embryonic lethality and gonadal defects. The BAF paralog, namely, barrier-to-autointegration factor 2 (BANF2), exhibits a testis-predominant expression pattern in both humans and mice. Unlike BAF, it may cause isolated male infertility. Therefore, we used the CRISPR/Cas9 system to generate Banf2-knockout mice to further study its function in spermatogenesis. Unexpectedly, knockout mice did not show any detectable abnormalities in histological structure of the testis, epididymis, ovary, and other tissues, and exhibited normal fertility, indicating that Banf2 is not essential for mouse spermatogenesis and fertility.