ADAPTIVE RESPONSE TO RADIATION-DAMAGE IN HUMAN-LYMPHOCYTES CONDITIONED WITH HYDROGEN-PEROXIDE AS MEASURED BY THE CYTOKINESIS-BLOCK MICRONUCLEUS TECHNIQUE
ADAPTIVE RESPONSE TO RADIATION-DAMAGE IN HUMAN-LYMPHOCYTES CONDITIONED WITH HYDROGEN-PEROXIDE AS MEASURED BY THE CYTOKINESIS-BLOCK MICRONUCLEUS TECHNIQUE
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DOI：
10.1016/0165-7992(93)90036-u
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发表时间：
1993-02-01
期刊：
影响因子：
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通讯作者：
CORTES, F
中科院分区：
文献类型：
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作者：
DOMINGUEZ, I;PANNEERSELVAM, N;CORTES, F
我们已经利用了微核法来评估适应性反应的人淋巴细胞从两个捐助者的条件与低剂量的过氧化氢前照射与1.5或3.0戈伊的X射线。预暴露于H2O2对辐射损伤的保护作用检测为微核在双核细胞中是显而易见的，在细胞允许恢复不同时期后，X射线曝光，虽然细胞挑战与3.0戈伊的X射线表现出适应性反应，在较晚的固定时间比那些暴露于较低的剂量（1.5戈伊）。我们提出这个协议作为一个有趣的替代单一的固定方法来评分染色体畸变中期的适应性反应的研究。
We have made use of the cytokinesis-block micronucleus method to evaluate the adaptive response in human lymphocytes from two donors conditioned with low doses of hydrogen peroxide before irradiation with either 1.5 or 3.0 Gy of X-rays. A protective effect of pre-exposure to H2O2 against radiation damage detected as micronuclei in binucleate cells was evident in cells allowed to recover for different periods after X-ray exposure, though cells challenged with 3.0 Gy of X-rays showed the adaptive response at later fixation time than those exposed to the lower dose (1.5 Gy). We propose this protocol as an interesting alternative to the single fixation method to score chromosomal aberrations at metaphase for the study of the adaptive response.