Involvement of strawberry notch homologue 1 in neurite outgrowth of cortical neurons
Involvement of strawberry notch homologue 1 in neurite outgrowth of cortical neurons
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DOI：
10.1111/dgd.12802
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发表时间：
2022-08
期刊：
影响因子：
4.6
通讯作者：
Munkhsoyol Erkhembaatar;Iroha Yamamoto;Fuduki Inoguchi;Kosuke Taki;S. Yamagishi;L. Delaney;Nishibe Mariko;T. Abe;H. Kiyonari;Carina Hanashima;H. Naka-Kaneda;D. Ihara;Y. Katsuyama
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文献类型：
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作者：
Munkhsoyol Erkhembaatar;Iroha Yamamoto;Fuduki Inoguchi;Kosuke Taki;S. Yamagishi;L. Delaney;Nishibe Mariko;T. Abe;H. Kiyonari;Carina Hanashima;H. Naka-Kaneda;D. Ihara;Y. Katsuyama
当轴突和树突生长的调节被改变时，神经元网络变得紊乱，这可能有助于精神疾病的发展。一些基因组分析表明草莓缺口同源物1（SBNO 1）突变和神经发育障碍之间的关系。然而，SBNO 1的功能尚未报道。在此，研究了小鼠大脑皮层发育过程中SBNO 1的表达模式。SBNO 1在皮质板中强烈表达，并且在出生后阶段其表达维持在低水平。在Neuro 2A培养细胞中基于CRISPR/Cas9的Sbno 1敲除显示出神经突生长延迟。构建了皮质神经元特异性条件性基因敲除小鼠，其导致皮质神经元中轴突束和树突的萎缩。因此，当突变时，SBNO 1是精神疾病的候选基因，如精神分裂症，正如人类基因组研究所建议的那样。
When the regulation of axonal and dendritic growth is altered, the neuronal network becomes disordered, which may contribute to the development of psychiatric disorders. Some genome analyses have suggested relationships between mutations in strawberry notch homologue 1 (SBNO1) and neurodevelopmental disorders. However, the function of SBNO1 has not yet been reported. Here, SBNO1 expression pattern during the development of the cerebral cortex in mice was examined. SBNO1 was strongly expressed in the cortical plate and its expression was maintained at a low level during the postnatal stage. CRISPR/Cas9‐based knockout of Sbno1 in Neuro2A cultured cells showed delayed growth of neurites. A cortical neuron‐specific conditional knockout mouse was constructed, which resulted in hypotrophy of axon bundles and dendrites in cortical neurons. Thus, when mutated, SBNO1 is a candidate gene for psychiatric diseases, such as schizophrenia, as suggested by human genome studies.